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Combined Chlorophyll Fluorescence and Transcriptomic Analysis Identifies the P3/P4 Transition as a Key Stage in Rice Leaf Photosynthetic Development
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Document Title
Combined Chlorophyll Fluorescence and Transcriptomic Analysis Identifies the P3/P4 Transition as a Key Stage in Rice Leaf Photosynthetic Development
Author
van Campen JC, Yaapar MN, Narawatthana S, Lehmeier C, Wanchana S, Thakur V, Chater C, Kelly S, Rolfe SA, Quick WP, Fleming AJ
Name from Authors Collection
Affiliations
University of Sheffield; CGIAR; International Rice Research Institute (IRRI); National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Universidad Nacional Autonoma de Mexico; University of Oxford; Ministry of Agriculture & Cooperatives - Thailand; University of Kansas
Type
Article
Source Title
PLANT PHYSIOLOGY
Year
2016
Volume
170
Issue
3
Page
1655-1674
Open Access
Bronze, Green Published
Publisher
OXFORD UNIV PRESS INC
DOI
10.1104/pp.15.01624
Format
Abstract
Leaves are derived from heterotrophic meristem tissue that, at some point, must make the transition to autotrophy via the initiation of photosynthesis. However, the timing and spatial coordination of the molecular and cellular processes underpinning this switch are poorly characterized. Here, we report on the identification of a specific stage in rice (Oryza sativa) leaf development (P3/P4 transition) when photosynthetic competence is first established. Using a combined physiological and molecular approach, we show that elements of stomatal and vascular differentiation are coordinated with the onset of measurable light absorption for photosynthesis. Moreover, by exploring the response of the system to environmental perturbation, we show that the earliest stages of rice leaf development have significant plasticity with respect to elements of cellular differentiation of relevance for mature leaf photosynthetic performance. Finally, by performing an RNA sequencing analysis targeted at the early stages of rice leaf development, we uncover a palette of genes whose expression likely underpins the acquisition of photosynthetic capability. Our results identify the P3/P4 transition as a highly dynamic stage in rice leaf development when several processes for the initiation of photosynthetic competence are coordinated. As well as identifying gene targets for future manipulation of rice leaf structure/function, our data highlight a developmental window during which such manipulations are likely to be most effective.
Funding Sponsor
Biotechnology and Biological Sciences Research Council [BB/J004065]; Malay Government studentship; Thai Government studentship; Biotechnology and Biological Sciences Research Council (International Rice Research Institute); Biotechnology and Biological Sciences Research Council (CASE studentship) [BB/J012610/1]; Biotechnology and Biological Sciences Research Council [1237544] Funding Source: researchfish
License
CC BY
Rights
Publisher
Publication Source
WOS