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Comparative analysis of the Spirulina platensis subcellular proteome in response to low- and high-temperature stresses: uncovering cross-talk of signaling components
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Document Title
Comparative analysis of the Spirulina platensis subcellular proteome in response to low- and high-temperature stresses: uncovering cross-talk of signaling components
Author
Kurdrid P, Senachak J, Sirijuntarut M, Yutthanasirikul R, Phuengcharoen P, Jeamton W, Roytrakul S, Cheevadhanarak S, Hongsthong A
Name from Authors Collection
Scopus Author ID
35097874300
Affiliations
National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); King Mongkuts University of Technology North Bangkok; King Mongkuts University of Technology Thonburi; King Mongkuts University of Technology North Bangkok; King Mongkuts University of Technology Thonburi
Type
Article
Source Title
PROTEOME SCIENCE
Year
2011
Volume
9
Page
-
Open Access
Green Published, gold
Publisher
BMC
DOI
10.1186/1477-5956-9-39
Format
Abstract
The present study focused on comparative proteome analyses of low-and high-temperature stresses and potential protein-protein interaction networks, constructed by using a bioinformatics approach, in response to both stress conditions. The data revealed two important points: first, the results indicate that low-temperature stress is tightly linked with oxidative stress as well as photosynthesis; however, no specific mechanism is revealed in the case of the high-temperature stress response. Second, temperature stress was revealed to be linked with nitrogen and ammonia assimilation. Moreover, the data also highlighted the cross-talk of signaling pathways. Some of the detected signaling proteins, e.g., Hik14, Hik26 and Hik28, have potential interactions with differentially expressed proteins identified in both temperature stress conditions. Some differentially expressed proteins found in the Spirulina protein-protein interaction network were also examined for their physical interactions by a yeast two hybrid system (Y2H). The Y2H results obtained in this study suggests that the potential PPI network gives quite reliable potential interactions for Spirulina. Therefore, the bioinformatics approach employed in this study helps in the analysis of phenomena where proteome analyses of knockout mutants have not been carried out to directly examine for specificity or cross-talk of signaling components.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
National Center for Genetic Engineering and Biotechnology (BIOTEC), Bangkok, Thailand
License
CC-BY
Rights
Authors
Publication Source
WOS