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Comparative proteomics for identification of reproductionrelated proteins in testes of the giant tiger shrimp penaeus monodon
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Metadata
Document Title
Comparative proteomics for identification of reproductionrelated proteins in testes of the giant tiger shrimp penaeus monodon
Author
Klinbunga S., Petkorn S., Phaonakrop N., Janpoom S., Prasertlux S., Rongmung P., Roytrakul S., Menasveta P., Khamnamtong B.
Name from Authors Collection
Scopus Author ID
35765811700
Affiliations
National Science and Technology Development Agency, National Center for Genetic Engineering and Biotechnology (BIOTEC), Khlong Nueng, Khlong LuangPathum Thani 12120, Thailand; Chulalongkorn University, Faculty of Science, Bangkok, 10330, Thailand; The Royal Society of Thailand, Academy of Science, Bangkok, 10300, Thailand
Type
Article
Source Title
Genetics of Aquatic Organisms
ISSN
24591831
Year
2020
Volume
4
Issue
2
Page
69-79
Open Access
Bronze
Publisher
Central Fisheries Research Inst
DOI
10.4194/2459-1831-v4_2_02
Abstract
Testicular proteome of wild and domesticated 14-and 18-month-old broodstock of the giant tiger shrimp Penaeus monodon was examined. Among 344 differentially expressed proteins identified, 11 proteins (e.g. p97/VCP-binding protein p135, lipoxygenase homology domains 1 and dipeptidyl-peptidase, accounting for 4.95% of proteins with known functions) were found in wild broodstock but not in domesticated broodstock while 152 (68.47%) proteins were commonly found in all groups of samples. Reproduction-related proteins such as vasa-like protein, Wee1-like protein kinase, serine/threonine protein kinase, mitogen activated protein kinase kinase 2, GTP-binding protein alpha subunit, seven membrane helix receptor, nuclear receptor subfamily 3, were identified. To examine a possible role of the signal transduction system in development of testes of P. monodon, the expression level of testicular serine/arginine rich-protein kinase 3 (PmSrpk3) mRNA of domesticated juveniles and broodstock was examined and it was significantly lower than that of wild P. monodon broodstock (P<0.05). PmSrpk3 expression was significantly induced by serotonin (P0.05) injection. The expression profiles of PmSrpk3 indicated reduced reproductive maturation of domesticated male P. monodon and exogenous administration of serotonin may be applied for promoting the testicular development of captive P. monodon. Published by Central Fisheries Research Institute (SUMAE) Trabzon, Turkey.
Keyword
Gene Expression | Penaeus monodon | Proteomics | Srpk3 | Testicular development
License
CC BY-NC-ND
Rights
Author
Publication Source
Scopus