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D-Lactic Acid Production from Sugarcane Bagasse by Genetically Engineered Saccharomyces cerevisiae
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Document Title
D-Lactic Acid Production from Sugarcane Bagasse by Genetically Engineered Saccharomyces cerevisiae
Author
Sornlek W, Sae-Tang K, Watcharawipas A, Wongwisansri S, Tanapongpipat S, Eurwilaichtr L, Champreda V, Runguphan W, Schaap PJ, Dos Santos VAPM
Name from Authors Collection
Scopus Author ID
6602764100
Affiliations
National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Wageningen University & Research; Mahidol University; National Science & Technology Development Agency - Thailand
Type
Article
Source Title
JOURNAL OF FUNGI
Year
2022
Volume
8
Issue
8
Open Access
Green Published, gold
Publisher
MDPI
DOI
10.3390/jof8080816
Format
Abstract
Lactic acid (LA) is a promising bio-based chemical that has broad applications in food, nutraceutical, and bioplastic industries. However, production of the D-form of LA (D-LA) from fermentative organisms is lacking. In this study, Saccharomyces cerevisiae harboring the D-lactate dehydrogenase (DLDH) gene from Leuconostoc mesenteroides was constructed (CEN.PK2_DLDH). To increase D-LA production, the CRISPR/Cas12a system was used for the deletion of gpd1, gpd2, and adh1 to minimize glycerol and ethanol production. Although an improved D-LA titer was observed for both CEN.PK2_DLDH Delta gpd and CEN.PK2_DLDH Delta gpd Delta adh1, growth impairment was observed. To enhance the D-LA productivity, CEN.PK2_DLDH Delta gpd was crossed with the weak acid-tolerant S. cerevisiae BCC39850. The isolated hybrid2 showed a maximum D-LA concentration of 23.41 +/- 1.65 g/L, equivalent to the improvement in productivity and yield by 2.2 and 1.5 folds, respectively. The simultaneous saccharification and fermentation using alkaline pretreated sugarcane bagasse by the hybrid2 led to an improved D-LA conversion yield on both the washed solid and whole slurry (0.33 and 0.24 g/g glucan). Our findings show the exploitation of natural yeast diversity and the potential strategy of gene editing combined with conventional breeding on improving the performance of S. cerevisiae for the production of industrially potent products.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Wageningen University; National Science and Technology Development Agency, Thailand [P-17-52777]
License
CC BY
Rights
D-Lactic Acid Production from Sugarcane Bagasse by Genetically Engineered Saccharomyces cerevisiae
Publication Source
WOS