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De novo chromosome-level assembly of the Centella asiatica genome
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Document Title
De novo chromosome-level assembly of the Centella asiatica genome
Author
Pootakham W., Naktang C., Kongkachana W., Sonthirod C., Yoocha T., Sangsrakru D., Jomchai N., U-thoomporn S., Romyanon K., Toojinda T., Tangphatsornruang S.
Name from Authors Collection
Scopus Author ID
57201312649
Scopus Author ID
56030033100
Scopus Author ID
36059124300
Scopus Author ID
57199231749
Affiliations
National Omics Center, National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand
Type
Article
Source Title
Genomics
ISSN
08887543
Year
2021
Volume
113
Issue
4
Page
2221-2228
Open Access
All Open Access, Hybrid Gold
Publisher
Academic Press Inc.
DOI
10.1016/j.ygeno.2021.05.019
Format
Abstract
Centella asiatica is a herbaceous, perennial species indigenous to India and Southeast Asia. C. asiatica possesses several medicinal properties: anti-aging, anti-inflammatory, wound healing and memory enhancing. The lack of available genomics resources significantly impedes the improvement of C. asiatica varieties through molecular breeding. Here, we combined the 10× Genomics linked-read technology and the long-range HiC technique to obtain the genome assembly. The final assembly contained nine pseudomolecules, corresponding to the haploid chromosome number in C. asiatica. These nine chromosomes covered 402,536,584 bases or 93.6% of the 430-Mb assembly. Comparative genomics analyses based on single-copy orthologous genes showed that C. asiatica and the common ancestor of Coriandrum sativum (coriander) and Daucus carota (carrot) diverged about 48 million years ago. This assembly provides a valuable reference genome for future molecular studies, varietal development through marker-assisted breeding and comparative genomics studies in C. asiatica. © 2021 The Author(s)
Keyword
10× Genomics | Centella asiatica | Chromosome-scale genome assembly | HiC | RNA-seq
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Department of Agriculture, Australian Government; National Science and Technology Development Agency
Funding Number
P-19-51263
License
N/A
Rights
N/A
Publication Source
Scopus