Development of a High-Accuracy Low-Cost and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

Abstract

This study presents the development of a portable fluorometer with a smartphone application designed to facilitate the early screening of chronic kidney and renal diseases by enabling the sensitive detection of urinary albumin. Utilizing a fluorescence-based aptasensor the device achieved a linear calibration curve (0.001�5 mg/mL) with a linearity of up to 0.98022 and a detection limit of 0.203 ?g/mL for human serum albumin (HSA). The analysis of 130 urine samples demonstrated comparable performance between this study抯 fluorometer a commercial fluorometer and the standard automated method. These findings validate the feasibility of the portable fluorometer and aptasensor combination as a reliable instrument for the sensitive and specific measurement of HSA in urine samples. Moreover the fluorometer抯 portability offers potential applications in portable point-of-care testing enhancing its utility in clinical settings for early disease screening. ? 2023 by the authors.