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Development of a Sensitive Self-Powered Glucose Biosensor Based on an Enzymatic Biofuel Cell
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Document Title
Development of a Sensitive Self-Powered Glucose Biosensor Based on an Enzymatic Biofuel Cell
Author
Chansaenpak K., Kamkaew A., Lisnund S., Prachai P., Ratwirunkit P., Jingpho T., Blay V., Pinyou P.
Name from Authors Collection
Affiliations
National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science ParkPathum Thani 12120, Thailand; School of Chemistry, Institute of Science, Suranaree University of Technology, 111 University AvenueNakhon Ratchasima 30000, Thailand; Department of Applied Chemistry, Faculty of Science and Liberal Arts, Rajamangala University of Technology Isan, 744, Suranarai Rd., Nakhon Ratchasima 30000, Thailand; Suranaree University of Technology, 111 University AvenueNakhon Ratchasima 30000, Thailand; Division of Biomaterials and Bioengineering, University of California San Francisco, 513 Parnassus Ave, San Francisco, CA 94143, USA
Type
Article
Source Title
Biosensors
ISSN
20796374
Year
2021
Volume
11
Issue
1
Open Access
Gold, Green
Publisher
NLM (Medline)
DOI
10.3390/bios11010016
Abstract
Biofuel cells allow for constructing sensors that leverage the specificity of enzymes without the need for an external power source. In this work, we design a self-powered glucose sensor based on a biofuel cell. The redox enzymes glucose dehydrogenase (NAD-GDH), glucose oxidase (GOx), and horseradish peroxidase (HRP) were immobilized as biocatalysts on the electrodes, which were previously engineered using carbon nanostructures, including multi-wall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO). Additional polymers were also introduced to improve biocatalyst immobilization. The reported design offers three main advantages: (i) by using glucose as the substrate for the both anode and cathode, a more compact and robust design is enabled, (ii) the system operates under air-saturating conditions, with no need for gas purge, and (iii) the combination of carbon nanostructures and a multi-enzyme cascade maximizes the sensitivity of the biosensor. Our design allows the reliable detection of glucose in the range of 0.1-7.0 mM, which is perfectly suited for common biofluids and industrial food samples.
License
CC BY
Rights
Author
Publication Source
Scopus