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Development of loop-mediated isothermal amplification (LAMP) assay using SYBR safe and gold-nanoparticle probe for detection of Leishmania in HIV patients
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Document Title
Development of loop-mediated isothermal amplification (LAMP) assay using SYBR safe and gold-nanoparticle probe for detection of Leishmania in HIV patients
Author
Ruang-areerate T., Sukphattanaudomchoke C., Thita T., Leelayoova S., Piyaraj P., Mungthin M., Suwannin P., Polpanich D., Tangchaikeeree T., Jangpatarapongsa K., Choowongkomon K., Siripattanapipong S.
Name from Authors Collection
Affiliations
Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, 10400, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand; Center for Research and Innovation, Faculty of Medical Technology, Mahidol University, Bangkok, 10700, Thailand; National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand; Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand
Type
Article
Source Title
Scientific Reports
ISSN
20452322
Year
2021
Volume
11
Issue
1
Open Access
Gold, Green
Publisher
Nature Research
DOI
10.1038/s41598-021-91540-5
Abstract
Asymptomatic leishmaniasis cases have continuously increased, especially among patients with HIV who are at risk to develop further symptoms of cutaneous and visceral leishmaniasis. Thus, early diagnosis using a simple, sensitive and reliable diagnostic assay is important because populations at risk mostly reside in rural communities where laboratory equipment is limited. In this study, the highly sensitive and selective determination of Leishmania infection in asymptomatic HIV patients was achieved using dual indicators (SYBR safe and gold-nanoparticle probe; AuNP-probe) in one-step LAMP method based on basic instruments. The assay can be simply evaluated under the naked eye due to clear interpretation of fluorescent emission of LAMP-SYBR safe dye-complex and colorimetric precipitate of specific AuNP-probes. The sensitivities and specificities of fluorescent SYBR safe dye and AuNP-probe indicators were equal, which were as high as 94.1 and 97.1%, respectively. Additionally, detection limits were 102 parasites/mL (0.0147 ng/µL), ten times more sensitivity than other related studies. To empower leishmaniasis surveillance, this inexpensive one-step SYBR safe and AuNP-LAMP assay is reliably fast and simple for field diagnostics to point-of-care settings, which can be set up in all levels of health care facilities including resource limited areas, especially in low to middle income countries. © 2021, The Author(s).
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Mahidol University; Kasetsart University Research and Development Institute; Faculty of Tropical Medicine, Mahidol University; Faculty of Science, Mahidol University; Faculty of Medicine Siriraj Hospital, Mahidol University; Department of Physiology, Mahidol University
License
CC BY
Rights
Author
Publication Source
Scopus