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Development of monoclonal antibodies for simple identification of Vibrio alginolyticus
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Document Title
Development of monoclonal antibodies for simple identification of Vibrio alginolyticus
Author
Sithigorngul W, Rengpipat S, Tansirisittikul A, Rukpratanporn S, Longyant S, Chaivisuthangkura P, Sithigorngul P
Name from Authors Collection
Affiliations
Srinakharinwirot University; Chulalongkorn University; Chulalongkorn University; Chulalongkorn University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
LETTERS IN APPLIED MICROBIOLOGY
ISSN
0266-8254
Year
2006
Volume
43
Issue
4
Page
436-442
Open Access
Bronze
Publisher
WILEY
DOI
10.1111/j.1472-765X.2006.01969.x
Format
Abstract
Aims: The present study was aimed to produce monoclonal antibodies (MAbs) for simple and specific identification of Vibrio alginolyticus infection in shrimp. Methods and Results: Mice were immunized with heat killed V. alginolyticus four times at 2-week intervals. The best response mouse was used for spleen donor in hybridoma production. Screening of hybridoma clones producing desired antibodies was performed by dot blotting against V. alginolyticus and other bacterial species, Western blotting and immunohistochemistry of infected shrimp tissues. Four groups of MAbs were obtained; the first group of MAbs demonstrated their limited specificity only to V. alginolyticus used for immunization, while the second and the third groups recognized all three isolates of V. alginolyticus used for testing. The fourth group of MAbs bound to all three isolates of V. alginolyticus and also recognized Vibrio parahaemolyticus, Vibrio harveyi, Vibrio fluvialis and Vibrio vulnificus but did not bind to Vibrio mimicus, Vibrio cholerae, Vibrio penaeicida and other bacterial species tested. MAbs in groups 1, 2 and 3 were able to use for the detection of bacterial infection in the tissues by means of immunohistochemistry. Conclusions: MAbs specific to V. alginolyticus was produced. These MAbs can be used for specific identification of the bacteria by simple 'dot blotting' method and immunohistochemistry. Significance and Impact of Study: This study demonstrated an immunological tool that can be used for simple and accurate identification of V. alginolyticus as well as for the diagnosis of V. alginolyticus infection in animals. This immunological tool can replace costly and laborious biochemical tests.
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WOS