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Differentially expressed genes in hemocytes of vibrio harveyi-challenged shrimp penaeus monodon
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Document Title
Differentially expressed genes in hemocytes of vibrio harveyi-challenged shrimp penaeus monodon
Author
Somboonwiwat K., Supungul P., Rimphanitchayakit V., Aoki T., Hirono I., Tassanakajon A.
Name from Authors Collection
Scopus Author ID
6508068998
Affiliations
Shrimp Molecular Biology and Genomics Laboratory, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand; Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo, 108-8477, Japan
Type
Article
Source Title
Journal of Biochemistry and Molecular Biology
ISSN
12258687
Year
2006
Volume
39
Issue
1
Page
26-36
Open Access
Bronze, Green
Publisher
The Biochemical Society of the Republic of Korea
DOI
10.5483/bmbrep.2006.39.1.026
Abstract
Differential Display PCR technique (DD-PCR) was used for the analysis of altered gene expression in hemocytes of Vibrio harveyi-infected Penaeus monodon. Forty-four combinations of arbitrary and oligo(dT) primers were used to screen for differentially expressed genes. A total of 79 differentially expressed bands could be identified from 33 primer combinations. These included 48 bands (61%) whose expression level increased and 31 bands (39%) decreased after V. harveyi challenge. Subsequently, fortyeight differential display fragments were successfully reamplifled and cloned. A total of 267 clones were randomly selected and sequenced. The sequence analysis showed that 85 (31%) out of 267 clones were matched with sequences in the GenBank database which represented 24 different genes with known functions. Among the known genes, glucose transporter 1, interferon-related developmental regulator 1, lysozyme, profilin, SERPINB3, were selected for further confirmation of their differentially expression patterns by real-time PCR. The results showed increasing in expression level of the selected genes in shrimp hemocytes after microbial challenge suggesting the involvement of such genes in bacterial response in shrimp. The anti-lipopolysaccharide factor type 3 (ALFPm3) gene, previously reported in P. monodon (Supungul et al., 2002) was found among the up-regulated genes but diversity due to amino acid changes was observed. Increase in ALFPm3 transcripts upon V. harveyi injection is in accordance with that found in the previous study.
License
CC BY-NC
Rights
Author
Publication Source
Scopus