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Differentially expressed genes in penaeus monodon hemocytes following infection with yellow head virus
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Document Title
Differentially expressed genes in penaeus monodon hemocytes following infection with yellow head virus
Author
Pongsomboon S., Tang S., Boonda S., Aoki T., Hirono I., Yasuike M., Tassanakajon A.
Name from Authors Collection
Affiliations
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand; Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo, Japan
Type
Article
Source Title
Journal of Biochemistry and Molecular Biology
ISSN
12258687
Year
2008
Volume
41
Issue
9
Page
670-677
Open Access
Gold, Green
Publisher
The Biochemical Society of the Republic of Korea
DOI
10.5483/bmbrep.2008.41.9.670
Abstract
A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeusjaponicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised five genes including cathepsin L-like cysteine peptidase, hypothetical proteins and unknown genes, was of particular interest because the transcripts increased rapidly (≤ 0.25 hours) and reached high expression levels in response to YHV injection. Microarray data were validated by realtime RT-PCR analyses of selected differentially expressed transcripts. In addition, comparative analysis of the hemocyte transcription levels of three of these genes between surviving and non-surviving shrimp revealed significantly higher expression levels in surviving shrimp.
License
CC BY-NC
Rights
Author
Publication Source
Scopus