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Effect of recombinant human erythroferrone protein on hepcidin gene (Hamp1) expression in hepg2 and huh7 cells
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Metadata
Document Title
Effect of recombinant human erythroferrone protein on hepcidin gene (Hamp1) expression in hepg2 and huh7 cells
Author
Than M.M., Koonyosying P., Ruangsuriya J., Junrungsee S., Uthaipibull C., Srichairatanakool S.
Name from Authors Collection
Affiliations
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand; Department of Biochemistry, University of Medicine, Mandalay, 05021, Myanmar; Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand; Protein-Ligand Engineering and Molecular Biology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani12120, Thailand
Type
Article
Source Title
Materials
ISSN
19961944
Year
2021
Volume
14
Issue
21
Open Access
Gold, Green
Publisher
MDPI
DOI
10.3390/ma14216480
Abstract
Iron is essential for all living organisms. It is strictly controlled by iron transporters, trans-ferrin receptors, ferroportin and hepcidin. Erythroferrone (ERFE) is an iron-regulatory hormone which is highly expressed in erythroblasts by erythropoietin (EPO) stimulation and osteoblasts independently of EPO by sequestering bone morphogenetic proteins and inhibiting hepatic hep-cidin expression. Although the hepcidin suppressive function of ERFE is known, its receptors still require investigation. Here, we aim to identify ERFE receptors on the HepG2 and Huh7 cells re-sponsible for ERFE. Recombinant ERFE (rERFE) was first produced in HEK293 cells transfected with pcDNA3.1 + ERFE, then purified and detected by Western blot. The liver cells were treated with an rERFE-rich medium of transfected HEK293 cells and a purified rERFE-supplemented medium at various time points, and hepcidin gene (Hamp1) expression was determined using qRT-PCR. The results show that 37-kD rERFE was expressed in HEK293 cells. Hamp1 was suppressed at 3 h and 6 h in Huh7 cells after rERFE treatments (p < 0.05), then restored to the original levels. Hamp1 was activated after treatment with purified rERFE for 24 h and 48 h. Together, these results reveal that ERFE suppressed Hamp1 expression in liver cells, possibly acting on membrane ERFE receptor, which in Huh7 cells was more sensitive to the ERFE concentrate. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keyword
erythroferrone | Hepcidin | Iron | Receptor | Recombinant protein
Funding Sponsor
Faculty of Medicine, Chiang Mai University
License
CC BY
Rights
Author
Publication Source
Scopus