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Efficient de novo production of bioactive cordycepin by Aspergillus oryzae using a food-grade expression platform
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Document Title
Efficient de novo production of bioactive cordycepin by Aspergillus oryzae using a food-grade expression platform
Author
Jeennor S. Anantayanon J. Panchanawaporn S. Chutrakul C. Vongsangnak W. Laoteng K.
Affiliations
Industrial Bioprocess Technology Research Team Functional Ingredients and Food Innovation Research Group National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Thailand Science Park Phahonyothin Road Khlong Nueng Pathum Thani Khlong Luang 12120 Thailand; Department of Zoology Faculty of Science Kasetsart University Bangkok 10900 Thailand; Omics Center for Agriculture Bioresources Food and Health Kasetsart University (OmiKU) Bangkok 10900 Thailand
Type
Article
Source Title
Microbial Cell Factories
ISSN
14752859
Year
2023
Volume
22
Issue
1
Open Access
All Open Access Gold
Publisher
BioMed Central Ltd
DOI
10.1186/s12934-023-02261-5
Abstract
Background: Cordycepin (3?-deoxyadenosine) is an important bioactive compound in medical and healthcare markets. The drawbacks of commercial cordycepin production using Cordyceps spp. include long cultivation periods and low cordycepin yields. To overcome these limitations and meet the increasing market demand the efficient production of cordycepin by the GRAS-status Aspergillus oryzae strain using a synthetic biology approach was developed in this study. Results: An engineered strain of A. oryzae capable of cordycepin production was successfully constructed by overexpressing two metabolic genes (cns1 and cns2) involved in cordycepin biosynthesis under the control of constitutive promoters. Investigation of the flexibility of carbon utilization for cordycepin production by the engineered A. oryzae strain revealed that it was able to utilize C6- C5- and C12-sugars as carbon sources with glucose being the best carbon source for cordycepin production. High cordycepin productivity (564.64 ? 9.59爉g/L/d) was acquired by optimizing the submerged fermentation conditions. Conclusions: This study demonstrates a powerful production platform for bioactive cordycepin production by A. oryzae using a synthetic biology approach. An efficient and cost-effective fermentation process for cordycepin production using an engineered strain was established offering a powerful alternative source for further upscaling. ? 2023 The Author(s).
License
CC BY
Rights
Authors
Publication Source
WOS