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Evidence of vertical transmission and tissue tropism of Streptococcosis from naturally infected red tilapia (Oreochromis spp.)
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Metadata
Document Title
Evidence of vertical transmission and tissue tropism of Streptococcosis from naturally infected red tilapia (Oreochromis spp.)
Author
Pradeep P.J.,Suebsing R.,Sirthammajak S.,Kampeera J.,Jitrakorn S.,Saksmerprome V.,Turner W.,Palang I.,Vanichviriyakit R.,Senapin S.,Jeffs A.,Kiatpathomchai W.,Withyachumanarnkul B.
Name from Authors Collection
Affiliations
Center of Excellence for Shrimp Molecular Biology and Technology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, 10400, Thailand; Bioengineering and Sensing Technology Laboratory, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Rd., Pathum Thani, Klong Neung, Klong Luang, 12120, Thailand; BIOTEC, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Rd., Klong Neung, Klong Luang, Pathum Thani, 12120, Thailand; Nam Sai Farms, 118 Bang Krabao, Ban Sang, Prachinburi, 25150, Thailand; Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand; Leigh Marine Laboratory, Institute of Marine Science, University of Auckland, Warkworth, New Zealand; Aquatic Animal Biotechnology Research Center, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani, 84100, Thailand
Type
Article
Source Title
Aquaculture Reports
ISSN
23525134
Year
2016
Volume
3
Open Access
All Open Access, Gold
Publisher
Elsevier B.V.
DOI
10.1016/j.aqrep.2015.12.002
Abstract
Streptococcosis is a highly problematic disease in the aquaculture of freshwater fishes, especially for tilapia. The possibility of vertical transmission of streptococcosis and the pattern of tissue tropism of this pathogen in various organs was examined in red tilapia (Oreochromis sp.). Healthy broodstock without any clinical signs of Streptococcus spp. were selected from a farm earlier reported to have the disease and a total of 10 pairs were forced spawned to provide samples of gametes and progeny for pathogen testing. A colorimetric LAMP assay was used to confirm whether the bacterial pathogens Streptococcus. agalactiae and Streptococcus. iniae was present in samples of milt, unfertilized eggs, fertilized eggs, and offspring at various stages of development, as well as internal organs of broodstock (reproductive organs, gill, liver, spleen, kidney and brain) as well as samples of water from culture systems. The majority of samples of milt (9/10) and unfertilized eggs (7/10) collected from the broodstock were infected with S. iniae at the time of spawning and was transmitted to all of their offspring. Nevertheless, when the same samples of gametes were analyzed for S. agalactiae, they were all found to be negative but the pathogen was found to be present in some 10-day-old larval offspring (4/10). However, when the pathogenic presence was analyzed from the reproductive organs of the parents, both S. agalactiae (11/20) and S. iniae (18/20) bacterium were common. Although, all broodstock were asymptomatic, almost all broodstock harboured the bacteria in many organs. Confirmation of vertical transmission of streptococcosis in tilapia means that intergenerational break cannot be used as a reliable and simple means of reducing or eliminating the prevalence of these difficult pathogens in aquaculture stock. © 2015 The Authors. Published by Elsevier B.V.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Mahidol University
License
CC BY-NC-ND
Rights
Elsevier B.V.
Publication Source
Scopus