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Exploring metabolic pathway alterations in obese fermented feces mediated by individual fruit extracts of Triphala components using untargeted metabolomics
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Document Title
Exploring metabolic pathway alterations in obese fermented feces mediated by individual fruit extracts of Triphala components using untargeted metabolomics
Author
Kwandee P. Somnuk S. Nakphaichit M. Wanikorn B. Roytrakul S. Tunsagool P.
Affiliations
Department of Biotechnology Faculty of Agro-Industry Kasetsart University Bangkok Thailand; Department of Sports and Health Sciences Faculty of Sport Science Kasetsart University Kamphaeng Saen Campus Nakhon Pathom Thailand; Functional Proteomics Technology Laboratory National Center for Genetic Engineering and Biotechnology Pathumthani Thailand
Type
Article
Source Title
Journal of King Saud University - Science
ISSN
10183647
Year
2024
Volume
36
Issue
4
Open Access
All Open Access Gold
Publisher
Elsevier B.V.
DOI
10.1016/j.jksus.2024.103115
Abstract
Objective: This work aimed to explore the modified pathways impacted by changes in fecal metabolites among female obese adults during the human gut model period both in the presence and absence of individual Triphala constituent fruit extract. Methods and results: The human gut model employed individual fruit extracts from Phyllanthus emblica Terminalia bellerica and T. chebula comparing them to a control group. Fermentation used fecal samples from female obese adults over 24 h. Metabolite extraction untargeted metabolomics and pathway analysis identified metabolic changes. Treatments with P. emblica extract T. bellerica extract and T. chebula extract revealed the statistical detection of 128 734 and 757 up-regulated metabolites respectively while 31 90 and 92 down-regulated metabolites were identified. Pathway analysis revealed that P. emblica extract primarily influenced vitamin B6 metabolism whereas the treatments with T. bellerica extract and T. chebula extract predominantly engaged glycine serine and threonine metabolism for metabolic regulation within the human gut model. Conclusion: By examining obese fecal metabolite changes and their association with metabolic pathway modulation through individual fruit extracts of Triphala constituents in a human gut model this study provides a comprehensive understanding of Triphala components� potential for managing obesity and its applications in the food industry. ? 2024 The Author(s)
Keyword
Feces | Metabolomics | Phyllanthus emblica | Terminalia bellerica | Terminalia chebula
License
CC BY-NC-ND
Rights
Authors
Publication Source
WOS