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Fabrication of Fish Scale-Based Gelatin Methacryloyl for 3D Bioprinting Application
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Document Title
Fabrication of Fish Scale-Based Gelatin Methacryloyl for 3D Bioprinting Application
Author
Pasanaphong K. Pukasamsombut D. Boonyagul S. Pengpanich S. Tawonsawatruk T. Wilairatanarporn D. Jantanasakulwong K. Rachtanapun P. Hemstapat R. Wangtueai S. Tanadchangsaeng N.
Affiliations
College of Biomedical Engineering Rangsit University Lak-Hok Pathumthani 12000 Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency Khlong Nueng Pathumthani 12120 Thailand; Department of Orthopaedics Faculty of Medicine Ramathibodi Hospital Mahidol University Ratchathewi Bangkok 10400 Thailand; Division of Packaging Technology School of Agro-Industry Faculty of Agro-Industry Chiang Mai University Mae Hia Muang Chiang-Mai 50100 Thailand; Department of Pharmacology Faculty of Science Mahidol University Ratchathewi Bangkok 10400 Thailand; College of Maritime Studies and Management Chiang Mai University Muang74000 Thailand
Type
Article
Source Title
Polymers
ISSN
20734360
Year
2024
Volume
16
Issue
3
Open Access
All Open Access Gold
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
DOI
10.3390/polym16030418
Abstract
Gelatin methacryloyl (GelMA) is an ideal bioink that is commonly used in bioprinting. GelMA is primarily acquired from mammalian sources; however the required amount makes the market price extremely high. Since garbage overflow is currently a global issue we hypothesized that fish scales left over from the seafood industry could be used to synthesize GelMA. Clinically the utilization of fish products is more advantageous than those derived from mammals as they lower the possibility of disease transmission from mammals to humans and are permissible for practitioners of all major religions. In this study we used gelatin extracted from fish scales and conventional GelMA synthesis methods to synthesize GelMA then tested it at different concentrations in order to evaluated and compared the mechanical properties and cell responses. The fish scale GelMA had a printing accuracy of 97% a swelling ratio of 482% and a compressive strength of about 85 kPa at a 10% w/v GelMA concentration. Keratinocyte cells (HaCaT cells) were bioprinted with the GelMA bioink to assess cell viability and proliferation. After 72 h of culture the number of cells increased by almost three-fold compared to 24 h as indicated by many fluorescent cell nuclei. Based on this finding it is possible to use fish scale GelMA bioink as a scaffold to support and enhance cell viability and proliferation. Therefore we conclude that fish scale-based GelMA has the potential to be used as an alternative biomaterial for a wide range of biomedical applications. ? 2024 by the authors.
Keyword
3D bioprinting | bioink | Cell proliferation | fish scale gelatin | hydrogel scaffold
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
WOS