• First Investigation of the Optimal Timing of Vaccination of Nile Tilapia (Oreochromis niloticus) Larvae against Streptococcus agalactiae

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Document Title

First Investigation of the Optimal Timing of Vaccination of Nile Tilapia (Oreochromis niloticus) Larvae against Streptococcus agalactiae

Author

Kumwan B. Bunnoy A. Chatchaiphan S. Kayansamruaj P. Dong H.T. Senapin S. Srisapoome P.

Affiliations

Laboratory of Aquatic Animal Health Management Department of Aquaculture Faculty of Fisheries Kasetsart University 50 Paholayothin Road Ladyao Chatuchak Bangkok 10900 Thailand; Center of Excellence in Aquatic Animal Health Management Department of Aquaculture Faculty of Fisheries Kasetsart University 50 Paholayothin Road Ladyao Chatuchak Bangkok 10900 Thailand; Department of Aquaculture Faculty of Fisheries Kasetsart University Bangkok 10900 Thailand; Aquaculture and Aquatic Resources Management Department of Food Agriculture and Bioresources School of Environment Resources and Development Asian Institute of Technology Pathum Thani 12120 Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani 12120 Thailand; Fish Health Platform Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) Faculty of Science Mahidol University Bangkok 10400 Thailand

Type

Article

Source Title

Vaccines

ISSN

2076393X

Year

2023

Volume

11

Issue

12

Open Access

All Open Access Gold

Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

DOI

10.3390/vaccines11121753

Abstract

To investigate early immune responses and explore the optimal vaccination periods Nile tilapia at 1 7 14 21 28 35 and 42 days after yolk sac collapse (DAYC) were immersed in formalin-killed Streptococcus agalactiae vaccine (FKV-SA). A specific IgM was first detected via ELISA in the 21 DAYC larvae (0.108 g) at 336 h after vaccination (hav) whereas in the 28� DAYC larvae (0.330�580 g) the specific IgM could be initially detected at 24 hav. qRT朠CR analysis of the TCR? CD4 MHCII? IgHM IgHT and IgHD genes in 21� DAYC larvae immunized with the FKV-SA immersion route for 24 168 and 336 hav revealed that the levels of most immune-related genes were significantly higher in the vaccinated larvae at all DAYCs than in the control larvae (p < 0.05) at 336 hav. Immunohistochemistry demonstrated stronger IgM signals in the gills head kidney and intestine tissues at 21 28 and 35 DAYC in all vaccinated larvae compared with the control. Interestingly at all DAYCs FKV-SA larvae exhibited significantly higher survival rates and an increased relative percent survival (RPS) than the control after challenge with viable S. agalactiae particularly in larvae that were immunized with FKV-SA at 168 and 336 hav (p < 0.05). ? 2023 by the authors.

Industrial Classification

N/A

Knowledge Taxonomy Level 1

N/A

Knowledge Taxonomy Level 2

N/A

Knowledge Taxonomy Level 3

N/A

License

CC BY

Rights

Authors

Publication Source

Scopus

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