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Genetic Aberration Analysis in Thai Colorectal Adenoma and Early-Stage Adenocarcinoma Patients by Whole-Exome Sequencing
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Document Title
Genetic Aberration Analysis in Thai Colorectal Adenoma and Early-Stage Adenocarcinoma Patients by Whole-Exome Sequencing
Author
Intarajak T, Udomchaiprasertkul W, Bunyoo C, Yimnoon J, Soonklang K, Wiriyaukaradecha K, Lamlertthon W, Sricharunrat T, Chaiwiriyawong W, Siriphongpreeda B, Sutheeworapong S, Kusonmano K, Kittichotirat W, Thammarongtham C, Jenjaroenpun P, Wongsurawat T, Nookaew I, Auewarakul C, Cheevadhanarak S
Name from Authors Collection
Affiliations
King Mongkuts University of Technology Thonburi; King Mongkuts University of Technology Thonburi; Chulabhorn Royal Academy; HRH Princess Chulabhorn College of Medical Science; King Mongkuts University of Technology Thonburi; Chulabhorn Royal Academy; HRH Princess Chulabhorn College of Medical Science; Chulabhorn Royal Academy; HRH Princess Chulabhorn College of Medical Science; Chulabhorn Royal Academy; HRH Princess Chulabhorn College of Medical Science; Chulabhorn Royal Academy; HRH Princess Chulabhorn College of Medical Science; Chulabhorn Research Institute; Chulabhorn Royal Academy; Chulabhorn Hospital; HRH Princess Chulabhorn College of Medical Science; Chulabhorn Research Institute; Chulabhorn Royal Academy; Chulabhorn Hospital; HRH Princess Chulabhorn College of Medical Science; King Mongkuts University of Technology Thonburi; King Mongkuts University of Technology Thonburi; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); University of Arkansas System; University of Arkansas Medical Sciences; Chalmers University of Technology; University of Arkansas System; University of Arkansas Medical Sciences
Type
Article
Source Title
CANCERS
Year
2019
Volume
11
Issue
7
Open Access
Green Published, Green Submitted, gold
Publisher
MDPI
DOI
10.3390/cancers11070977
Format
Abstract
Colorectal adenomas are precursor lesions of colorectal adenocarcinoma. The transition from adenoma to carcinoma in patients with colorectal cancer (CRC) has been associated with an accumulation of genetic aberrations. However, criteria that can screen adenoma progression to adenocarcinoma are still lacking. This present study is the first attempt to identify genetic aberrations, such as the somatic mutations, copy number variations (CNVs), and high-frequency mutated genes, found in Thai patients. In this study, we identified the genomic abnormality of two sample groups. In the first group, five cases matched normal-colorectal adenoma-colorectal adenocarcinoma. In the second group, six cases matched normal-colorectal adenomas. For both groups, whole-exome sequencing was performed. We compared the genetic aberration of the two sample groups. In both normal tissues compared with colorectal adenoma and colorectal adenocarcinoma analyses, somatic mutations were observed in the tumor suppressor gene APC (Adenomatous polyposis coli) in eight out of ten patients. In the group of normal tissue comparison with colorectal adenoma tissue, somatic mutations were also detected in Catenin Beta 1 (CTNNB1), Family With Sequence Similarity 123B (FAM123B), F-Box And WD Repeat Domain Containing 7 (FBXW7), Sex-Determining Region Y-Box 9 (SOX9), Low-Density Lipoprotein Receptor-Related Protein 5 (LRP5), Frizzled Class Receptor 10 (FZD10), and AT-Rich Interaction Domain 1A (ARID1A) genes, which are involved in the Wingless-related integration site (Wnt) signaling pathway. In the normal tissue comparison with colorectal adenocarcinoma tissue, Kirsten retrovirus-associated DNA sequences (KRAS), Tumor Protein 53 (TP53), and Ataxia-Telangiectasia Mutated (ATM) genes are found in the receptor tyrosine kinase-RAS (RTK-RAS) signaling pathway and p53 signaling pathway, respectively. These results suggest that APC and TP53 may act as a potential screening marker for colorectal adenoma and early-stage CRC. This preliminary study may help identify patients with adenoma and early-stage CRC and may aid in establishing prevention and surveillance strategies to reduce the incidence of CRC.
Funding Sponsor
Chulabhorn Royal Academy; King Mongkut's University of technology Thonburi; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM125503] Funding Source: NIH RePORTER
License
CC BY
Rights
Authors
Publication Source
WOS