-
Genetic loci regulating cadmium content in rice grains
- Back
Document Title
Genetic loci regulating cadmium content in rice grains
Author
Norton G.J.,Travis A.,Ruang-areerate P.,Nicol G.W.,Adeosun A.A.,Hossain M.,Islam M.R.,Douglas A.,Price A.H.
Name from Authors Collection
Affiliations
School of Biological Sciences, University of Aberdeen, Aberdeen, AB24 3UU, United Kingdom; National Omics Center, National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand; Laboratoire Ampère, École Centrale de Lyon, Université de Lyon, 36 avenue Guy de Collongue, Ecully Cedex, 69134, France; Department of Soil Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
Type
Article
Source Title
Euphytica
ISSN
00142336
Year
2021
Volume
217
Issue
3
Open Access
All Open Access, Hybrid Gold, Green
Publisher
Springer Science and Business Media B.V.
DOI
10.1007/s10681-020-02752-1
Abstract
It has been estimated that up to 90% of human exposure to cadmium is through food, and that cadmium within rice grains can be a major contributor to that dietary source. In this study genome wide association mapping was conducted on the Bengal and Assam Aus Panel (BAAP) of rice to identify quantitative trait loci and candidate genes for lowering grain cadmium. Field experiments were conducted over two years under two different irrigation systems: continually flooded and alternate wetting and drying (AWD). There was significant effects of water treatment, genotype, and genotype by water treatment interaction. Importantly, AWD increased grain cadmium, on average, by 49.6% and 108.8% in year 1 and 2 respectively. There was between 4.6 and 28 fold variation in cadmium concentration. A total of 58 QTLs were detected but no loci are clearly specific to one water regime despite approximately 20% of variation attributable to genotype by water regime interaction. A number of QTLs were consistent across most water treatments and years. These included QTLs on chromosome 7 (7.23–7.61, 8.93–9.04, and 29.12–29.14 Mbp), chromosome 5 (8.66–8.72 Mbp), and chromosome 9 (11.46–11.64 Mbp). Further analysis of the loci on chromosome 7 (8.93–9.04 Mbp), identified the candidate gene OsNRAMP1, where cultivars with a deletion upstream of the gene had higher concentrations of cadmium compared to the cultivars that did not have the deletion. The distribution of alleles within the BAAP suggest this QTL is easily detected in this population because it is composed of aus cultivars. Local genome cluster analysis suggest high Cd alleles are uncommon, but should be avoided in breeding. © 2021, The Author(s).
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Biotechnology and Biological Sciences Research Council; University of Aberdeen
License
CC BY
Rights
Author
Publication Source
Scopus