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Genetic modification of essential fatty acids biosynthesis in Hansenula polymorpha
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Document Title
Genetic modification of essential fatty acids biosynthesis in Hansenula polymorpha
Author
Laoteng K, Ruenwai R, Tanticharoen M, Cheevadhanarak S
Name from Authors Collection
Affiliations
King Mongkuts University of Technology North Bangkok; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); King Mongkuts University of Technology North Bangkok; King Mongkuts University of Technology Thonburi; King Mongkuts University of Technology North Bangkok; King Mongkuts University of Technology Thonburi
Type
Article
Source Title
FEMS MICROBIOLOGY LETTERS
ISSN
0378-1097
Year
2005
Volume
245
Issue
1
Page
169-178
Open Access
Bronze
Publisher
OXFORD UNIV PRESS
DOI
10.1016/j.femsle.2005.03.006
Format
Abstract
The Delta(6)-desaturase gene isoform II involved in the formation of gamma-linolenic acid (GLA) was identified from Mucor rouxii. To study the possibility of alteration of the synthetic pathway of essential fatty acids in the methylotrophic yeast, Hansenula polymorpha, the cloned gene of M. rouxii under the control of the methanol oxidase (MOX) promoter of H. polymorpha, was used for genetic modification of this yeast. Changes in flux through the n-3 and n-6 pathways in the transgenic yeast were observed. The proportion of GLA varied dramatically depending on the growth temperature and media composition. This can be explained by the effects of either substrate availability or enzymatic activity. In addition to the potential application for manipulating the fatty acid profile, this study provides an attractive model system of H. polymorpha for investigating the deviation of fatty acid metabolism in eukaryotes. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Publication Source
WOS