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Genomic diversity of large-plaque-forming podoviruses infecting the phytopathogen Ralstonia solanacearum
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Metadata
Document Title
Genomic diversity of large-plaque-forming podoviruses infecting the phytopathogen Ralstonia solanacearum
Author
Kawasaki T., Narulita E., Matsunami M., Ishikawa H., Shimizu M., Fujie M., Bhunchoth A., Phironrit N., Chatchawankanphanich O., Yamada T.
Name from Authors Collection
Scopus Author ID
35280829300
Affiliations
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, 739-8530, Japan; University of Jember, Jember, 68121, Indonesia; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand
Type
Article
Source Title
Virology
ISSN
00426822
Year
2016
Volume
492
Page
73-81
Open Access
Bronze
Publisher
Academic Press Inc.
DOI
10.1016/j.virol.2016.02.011
Abstract
The genome organization, gene structure, and host range of five podoviruses that infect Ralstonia solanacearum, the causative agent of bacterial wilt disease were characterized. The phages fell into two distinctive groups based on the genome position of the RNA polymerase gene (i.e., T7-type and φKMV-type). One-step growth experiments revealed that φRSB2 (a T7-like phage) lysed host cells more efficiently with a shorter infection cycle (ca. 60 min corresponding to half the doubling time of the host) than φKMV-like phages such as φRSB1 (with an infection cycle of ca. 180 min). Co-infection experiments with φRSB1 and φRSB2 showed that φRSB2 always predominated in the phage progeny independent of host strains. Most phages had wide host-ranges and the phage particles usually did not attach to the resistant strains; when occasionally some did, the phage genome was injected into the resistant strain's cytoplasm, as revealed by fluorescence microscopy with SYBR Gold-labeled phage particles. © 2016 Elsevier Inc.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Japan Society for the Promotion of Science; Japan Science and Technology Agency
License
CC BY or CC BY-NC-ND
Rights
Elsevier B.V.
Publication Source
Scopus