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Genomic Insight and Optimization of Astaxanthin Production from a New Rhodotorula sp. CP72-2
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Document Title
Genomic Insight and Optimization of Astaxanthin Production from a New Rhodotorula sp. CP72-2
Author
Kingkaew E., Tedsree N., Phuengjayaem S., Rojsitthisak P., Sritularak B., Thitikornpong W., Thompho S., Mhuantong W., Tanasupawat S.
Type
Article
Source Title
Fermentation
ISSN
23115637
Year
2023
Volume
9
Issue
6
Open Access
All Open Access, Gold
Publisher
MDPI
DOI
10.3390/fermentation9060501
Format
Abstract
Astaxanthin is a carotenoid pigment extensively used in various industries. Rhodotorula sp. CP72-2, isolated from Calotropis gigantea, showed potential astaxanthin production. In this study, strain CP72-2 was identified as a putative new species in the genus Rhodotorula based on the 26S rRNA gene sequence (98% identity). It was first used as the microbial source for producing astaxanthin. Strain CP72-2 was screened for its astaxanthin production and was identified and quantified by High-Performance Liquid Chromatography (HPLC), Liquid Chromatography-Mass Spectrometry (LC-MS), and UV-Vis spectrophotometer. After a screening of astaxanthin production, various carbon sources, pH, temperature, and incubation period were evaluated for their effect on the astaxanthin production of strain CP72-2. Among the several experimental factors, the most efficient conditions for astaxanthin production were glucose (50 g/L), pH 4.5, 25 ?C, and three days of cultivation. The assembly genome of strain CP72-2 has a total length of 21,358,924 bp and a GC content of 64.90%. The putative candidate astaxanthin biosynthesis-associated genes (i.e., CrtE, CrtYB, CrtI, CrtS, CrtR, CrtW, CrtO, and CrtZ) were found. This research presents the first report on the production and optimization of astaxanthin from strain CP72-2 and its genome analysis, focusing on the biotechnological potential of the astaxanthin producer. ? 2023 by the authors.
License
CC BY
Rights
Authors
Publication Source
WOS