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Hatakabb, a herbal extract, contains pyrogallol as the novel mediator inhibiting LPS-induced TNF-alpha production by NF-kappa B inactivation and HMOX-1 upregulation
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Document Title
Hatakabb, a herbal extract, contains pyrogallol as the novel mediator inhibiting LPS-induced TNF-alpha production by NF-kappa B inactivation and HMOX-1 upregulation
Author
Chantarasakha K, Asawapanumas T, Suntivich R, Panya A, Phonsatta N, Thiennimitr P, Laoteng K, Tepaamorndech S
Name from Authors Collection
Affiliations
National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Chulalongkorn University; Chiang Mai University; Chiang Mai University; Chiang Mai University; Chulalongkorn University
Type
Article
Source Title
JOURNAL OF FUNCTIONAL FOODS
ISSN
1756-4646
Year
2022
Volume
90
Page
-
Open Access
gold
Publisher
ELSEVIER
DOI
10.1016/j.jff.2022.104992
Format
Abstract
Hatakabb is a herbal extract traditionally consumed to ameliorate inflammation from pharynx infections. However, the anti-inflammatory function of Hatakabb was not investigated. This study demonstrated the anti-inflammatory potential, and underlying mechanism of Hatakabb, and its functional ingredient. In LPS-treated macrophages, Hatakabb treatment attenuated TNF-alpha production. A decrease in TNF-alpha, IL-6, and COX-2 expression was detected. In regulatory pathway analysis, Hatakabb suppressed I kappa B-alpha phosphorylation in accordance with a decrease in I kappa B-alpha degradation, and nucleus NF-kappa B translocation, while enhanced NF-kappa B phosphorylation. In addition, MAPK phosphorylation was enhanced in association with HMOX-1 upregulation. Our metabolomic analysis showed gallic acid, and pyrogallol contained in the anti-inflammatory subfraction. Compared to gallic acid, pyrogallol strongly diminished TNF-alpha production. Significant changes in inflammatory gene expression, and regulatory protein phosphorylation were responded to pyrogallol in similar to Hatakabb treatment. Our study, for the first time, reveals the anti-inflammatory effect, and mechanism of Hatakabb in which pyrogallol is the novel mediator inhibiting LPS-induced inflammation.
Keyword
Anti-inflammation | Hatakabb | HMOX-1 | NF-kappa B | Pyrogallol | TNF-alpha
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
NSTDA [P2050200]
Publication Source
WOS