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Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring
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Document Title
Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring
Author
Abdulrachman D, Thongkred P, Kocharin K, Nakpathom M, Somboon B, Narumol N, Champreda V, Eurwilaichitr L, Suwanto A, Nimchua T, Chantasingh D
Name from Authors Collection
Affiliations
National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); University Katolik Indonesia Atma Jaya; National Science & Technology Development Agency - Thailand; National Metal & Materials Technology Center (MTEC)
Type
Article
Source Title
BMC BIOTECHNOLOGY
ISSN
1472-6750
Year
2017
Volume
17
Page
-
Open Access
Green Published, gold
Publisher
BIOMED CENTRAL LTD
DOI
10.1186/s12896-017-0334-9
Format
Abstract
Background: Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-polygalacturonase (EndoPG) from Aspergillus aculeatus produced in Pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation. Results: The recombinant EndoPG exhibited a specific activity of 1892.08 U/mg on citrus pectin under the optimal condition at 50 degrees C, pH 5.0 with a V-max and K-m of 65,451.35 mu mol/min/mL and 15.14 mg/mL, respectively. A maximal activity of 2408.70 +/- 26.50 U/mL in the culture supernatant was obtained by high cell density batch fermentation, equivalent to a 4.8 times greater yield than that from shake-flask culture. The recombinant enzyme was shown to be suitable for application as a bioscouring agent, in which the wettability of cotton fabric was increased by treatment with enzyme at 300 U/mL scouring solution at 40 degrees C, pH 5.0 for 1 h. The bio-scoured fabric has comparable wettability to that obtained by conventional chemical scouring, but has higher tensile strength. Conclusion: The work has demonstrated for the first time functions of A. aculeatus EndoPG on bioscouring in eco-textile processing. EndoPG alone was shown to possess effective scouring activity. High expression level and homogeneity could be achieved in bench-scale bioreactor.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Thailand [P-13-50429]
License
CC BY
Rights
Authors
Publication Source
WOS