-
Hik28-dependent and Hik28-independent ABC transporters were revealed by proteome-wide analysis of ΔHik28 under combined stress
- Back
Document Title
Hik28-dependent and Hik28-independent ABC transporters were revealed by proteome-wide analysis of ΔHik28 under combined stress
Author
Kurdrid P., Yutthanasirikul R., Saree S., Senachak J., Saelee M., Hongsthong A.
Name from Authors Collection
Scopus Author ID
35097874300
Scopus Author ID
23398533200
Affiliations
Biosciences and System Biology Team, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at King Mongkut’s University of Technology Thonburi, Bangkok, 10150, Thailand; Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, 10150, Thailand
Type
Article
Source Title
BMC Molecular and Cell Biology
ISSN
26618850
Year
2022
Volume
23
Issue
1
Open Access
Gold, Green
Publisher
BioMed Central Ltd
DOI
10.1186/s12860-022-00421-w
Format
Abstract
Synechocystis histidine kinase, Sll0474: Hik28, a signal protein in a two-component signal transduction system, plays a critical role in responding to a decrease in growth temperature and is also involved in nitrogen metabolism. In the present study, under combined stress from non-optimal growth temperature and nitrogen depletion, a comparative proteomic analysis of the wild type (WT) and a deletion mutant (MT) of Synechocystis histidine kinase, Sll0474: Hik28, in a two-component signal transduction system identified the specific groups of ABC transporters that were Hik28-dependent, e.g., the iron transporter, and Hik28-independent, e.g., the phosphate transporter. The iron transporter, AfuA, was found to be upregulated only in the WT strain grown under the combined stress of high temperature and nitrogen depletion. Whereas, the expression level of the phosphate transporter, PstS, was increased in both the WT and MT strains. Moreover, the location in the genome of the genes encoding Hik28 and ABC transporters in Synechocystis sp. PCC6803 were analyzed in parallel with the comparative proteomic data. The results suggested the regulation of the ABC transporters by the gene in a two-component system located in an adjacent location in the genome. © 2022, The Author(s).
Keyword
ABC transporter | Arthrospira platensis | Article | ATP-Binding Cassette Transporters | bacterial growth | bacterial strain | Carbon | cell growth | Chlorophyll | comparative proteomics | cyanobacterium | Deletion mutant | digestion | enzyme activity | Genetics | Genome | Genome and Cyanobacteria | genomic DNA | glucoside | growth rate | High temperature | histidine kinase | Iron | iron transporter | Liquid chromatography-mass spectrometry | membrane protein | Metabolic pathway | Metabolism | nitrogen | nitrogen metabolism | nonhuman | oxidoreductase | oxygen evolution | Peptide | Phosphate Transport Proteins | phosphate transporter | Photosynthesis | physiological stress | protein analysis | protein degradation | protein expression | protein histidine kinase | protein protein interaction | Proteome | Proteomics | quantitative analysis | regulator protein | Response mechanism | Saccharomyces cerevisiae | signal transduction | Stress response | Synechocystis | tandem mass spectrometry | temperature | time of flight mass spectrometry | Trypsin | two hybrid system | unclassified drug | urea | Yeast
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency; King Mongkut's University of Technology Thonburi; National Center for Genetic Engineering and Biotechnology
License
CC BY
Rights
Author
Publication Source
Scopus