• Human-aided dispersal and population bottlenecks facilitate parasitism escape in the most invasive mosquito species

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Document Title

Human-aided dispersal and population bottlenecks facilitate parasitism escape in the most invasive mosquito species

Author

Girard M., Martin E., Vallon L., Tran Van V., Da Silva Carvalho C., Sack J., Bontemps Z., Balteneck J., Colin F., Duval P., Malassign? S., Hennessee I., Vizcaino L., Romer Y., Dada N., Ly Huynh Kim K., Huynh Thi Thuy T., Bellet C., Lambert G., Nantenaina Raharimalala F., Jupatanakul N., Goubert C., Boulesteix M., Mavingui P., Desouhant E., Luis P., Cazabet R., Hay A.-E., Valiente Moro C., Minard G.

Affiliations

Department of Biology, School of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, 10520, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Pathum Thani, Khlong Luang, 12120, Thailand; Thailand Bioresource Research Center (TBRC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Pathum Thani, Khlong Luang, 12120, Thailand; Department of Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok, 26120, Thailand; Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China; Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand; Actinobacterial Research Unit, School of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, 10520, Thailand

Type

Article

Source Title

Scientific Reports

ISSN

20452322

Year

2024

Volume

14

Issue

1

Open Access

All Open Access, Gold

Publisher

Nature Research

DOI

10.1038/s41598-024-60744-w

Abstract

Pradimicin U is a new dihydrobenzo[a]naphthacenequinone compound found to be active on a screen designed to investigate compounds with antimicrobial activity, produced by the actinomycete designated strain FMUSA5-5T. The strain was isolated from a bio-fertilizer of Musa spp. collected from Suphanburi province, Thailand. The chemotaxonomic characteristics and 16S rRNA gene analysis revealed that strain FMUSA5-5T is a member of the genus Nonomuraea. Low genome-based taxonomic criteria, average nucleotide identity (ANI) (82.8–88.3%), average amino-acid identity (AAI) (79.4–87.3%), and digital DNA–DNA hybridization (dDDH) (29.5–38.5%) values and several phenotypic differences between strain FMUSA5-5T and its closest type strains of the genus Nonomuraea indicated that strain FMUSA5-5T represents a novel species of the genus Nonomuraea and the name Nonomuraea composti sp. nov. is proposed for the strain. The crude extract from the culture broth of strain FMUSA5-5T displayed promising antimicrobial activity against several pathogens and led to the isolation of a novel secondary metabolite, pradimicin U. Interestingly, this compound displayed a broad spectrum of biological activities such as antimalarial activity against Plasmodium falciparum K1 (IC50 value = 3.65 ?g/mL), anti-Mycobacterium tuberculosis H37Ra (MIC value = 25.0 ?g/mL), anti-Alternaria brassicicola BCC 42724 (MIC value = 25.0 ?g/mL), anti-Bacillus cereus ATCC 11778 and anti-Staphylococcus aureus ATCC 29213 (MIC values = 6.25 and 1.56 ?g/mL, respectively). Moreover, the compound possessed strong anti-human small cell lung cancer (NCI-H187) activity with IC50 value of 5.69 ?g/mL, while cytotoxicity against human breast cancer (MCF-7) and Vero cells was very weak (IC50 values of 52.49 and 21.84 ?g/mL, respectively). ? The Author(s) 2024.

License

CC BY

Rights

Authors

Publication Source

WoS

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