-
Human serum enhances the proliferative capacity and immunomodulatory property of MSCs derived from human placenta and umbilical cord
- Back
Metadata
Document Title
Human serum enhances the proliferative capacity and immunomodulatory property of MSCs derived from human placenta and umbilical cord
Author
Thaweesapphithak S., Tantrawatpan C., Kheolamai P., Tantikanlayaporn D., Roytrakul S., Manochantr S.
Name from Authors Collection
Affiliations
Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand; Center of Excellence in Stem Cell Research, Thammasat University, Pathumthani, 12120, Thailand; Genome Institute, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, Thailand
Type
Article
Source Title
Stem Cell Research and Therapy
ISSN
17576512
Year
2019
Volume
10
Issue
1
Open Access
Gold, Green
Publisher
BioMed Central Ltd.
DOI
10.1186/s13287-019-1175-3
Abstract
Background: Mesenchymal stromal cells (MSCs) are considered potential candidates that hold great promise in the treatment of immune-related diseases. For therapeutic applications, it is necessary to isolate and expand MSCs with procedures complying with good manufacturing practice (GMP). Recent studies reported the use of human serum (HS) instead of fetal bovine serum (FBS) for the expansion of bone marrow-derived MSCs. Nevertheless, there are only limited data on HS as an alternative to FBS for the isolation and expansion of umbilical (UC-MSCs) and placenta-derived MSCs (PL-MSCs). In this study, we evaluate the effect of HS compared to FBS on the proliferative and immunosuppressive capacities of these MSCs. Methods: PL-MSCs and UC-MSCs were isolated and cultured in HS- or FBS-supplemented media. The MSC characteristics, including morphology, immunophenotype, and differentiation ability, were verified. The proliferative and immunosuppressive capacities were also examined. In addition, the proliferative-enhancing factors in both sera were explored using proteomic analysis. Results: PL-MSCs and UC-MSCs proliferated faster in HS-supplemented medium than in equivalent levels of FBS-supplemented medium. Adipogenic and osteogenic differentiations occurred at nearly identical levels in HS- and FBS-supplemented media. Interestingly, MSCs cultured in HS-supplemented medium had a similar immunosuppressive effect as MSCs cultured in FBS-supplemented medium. Proteomic analysis revealed that Con-A binding glycoproteins with a molecular weight > 100 kDa in FBS could significantly enhance MSC proliferation. In contrast, the proliferative enhancing factors in HS were found in the Con-A non-binding fraction and WGA binding fraction with a molecular weight > 100 kDa. Conclusions: Taken together, our results suggest applications for the use of HS instead of FBS for the isolation and expansion of PL-MSCs and UC-MSCs for cell therapy in the future. Furthermore, this study identifies factors in HS that are responsible for its proliferative and immunosuppressive effects and might thus lead to the establishment of GMPs for the therapeutic use of MSCs. © 2019 The Author(s).
Keyword
Con-A | Human serum | immunosuppression | Mesenchymal stromal cell | Placenta | Umbilical cord | WGA
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Thailand Research Fund; Thammasat University
License
CC BY
Rights
Author
Publication Source
Scopus