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Identical Hik-Rre systems are involved in perception and transduction of salt signals and hyperosmotic signals but regulate the expression of individual genes to different extents in Synechocystis
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Document Title
Identical Hik-Rre systems are involved in perception and transduction of salt signals and hyperosmotic signals but regulate the expression of individual genes to different extents in Synechocystis
Author
Shumskaya MA, Paithoonrangsarid K, Kanesaki Y, Los DA, Zinchenko VV, Tanticharoen M, Suzuki I, Murata N
Name from Authors Collection
Scopus Author ID
6507201719
Affiliations
National Institutes of Natural Sciences (NINS) - Japan; National Institute for Basic Biology (NIBB); Russian Academy of Sciences; Timiryazev Institute of Plant Physiology; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Lomonosov Moscow State University; Graduate University for Advanced Studies - Japan
Type
Article
Source Title
JOURNAL OF BIOLOGICAL CHEMISTRY
Year
2005
Volume
280
Issue
22
Page
21531-21538
Open Access
Green Published, hybrid
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI
10.1074/jbc.m412174200
Format
Abstract
In previous studies, we characterized five histidine kinases (Hiks) and the cognate response regulators (Rres) that control the expression of similar to 70% of the hyperosmotic stress-inducible genes in the cyanobacterium Synechocystis sp. PCC 6803. In the present study, we screened a gene knock-out library of Rres by RNA slot-blot hybridization and with a genome-wide DNA microarray and identified three Hik-Rre systems, namely, Hik33-Rre31, Hik10-Rre3, and Hik16-Hik41-Rre17, as well as another system that included Rre1, that were involved in perception of salt stress and transduction of the signal. We found that these Hik-Rre systems were identical to those that were involved in perception and transduction of the hyperosmotic stress signal. We compared the induction factors of the salt stress- and hyperosmotic stress- inducible genes that are located downstream of each system and found that these genes responded to the two kinds of stress to different respective extents. In addition, the Hik33-Rre31 system regulated the expression of genes that were specifically induced by hyperosmotic stress, whereas the system that included Rre1 regulated the expression of one or two genes that were specifically induced either by salt stress or by hyperosmotic stress. Our observations suggest that the perception of salt and hyperosmotic stress by the Hik-Rre systems is complex and that salt stress and hyperosmotic stress are perceived as distinct signals by the Hik-Rre systems.
License
Copyright
Rights
ASBMB
Publication Source
WOS