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Identification of cassava black stem and root rot agents in Thailand
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Document Title
Identification of cassava black stem and root rot agents in Thailand
Author
Sangpueak R., Duchanee S., Saengchan C., Papathoti N.K., Hoang N.H., Thanh T.L., Phansak P., Buensanteai N.
Affiliations
Suranaree University of Technology, School of Crop Production Technology, Institute of Agricultural Technology, Nakhon Ratchasima, 30000, Thailand; National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand; Can Tho University, College of Agriculture, Department of Plant Protection, Can Tho, 900000, Viet Nam; Nakhon Phanom University, Faculty of Science, Division of Biology, Nakhon Phanom, 48000, Thailand
Type
Article
Source Title
Chilean Journal of Agricultural Research
ISSN
7185820
Year
2023
Volume
83
Issue
1
Page
70-82
Open Access
All Open Access, Gold
Publisher
Instituto de Investigaciones Agropecuarias, INIA
DOI
10.4067/S0718-58392023000100070
Format
Abstract
Cassava (Manihot esculenta Crantz) black stem and root rot (BSRR) caused by complex fungi is one of the most serious fungal disease of cassava in Thailand. The objective of this study was to identify the causal agent of BSRR disease of cassava in Nakhon Ratchasima province, Thailand. From June 2015 to May 2018, the outbreak areas in three districts of Nakhon Ratchasima province were surveyed for cassava plants with the characteristic symptoms. From 1800 diseased samples, 139 fungal pathogens were isolated and separated by morphological traits. Among them, 33 fungal isolates were subsequently tested for pathogenicity on detached stems and roots of CMR 43-08-89, a susceptible cassava cultivar, and proved to be pathogenic with different levels of aggressiveness. Next, PCR amplification of DNA of eight pathogenic isolates with high aggressiveness using two sets of universal primers ITS1/ITS4 and Ef1-688F/Ef1-1251R, revealed that the TEF1-? gene region could be used for the identification and classification at the species level. The analysis results fit well with that of the morphological studies on growth and colonial characteristics of the eight isolates. Based on the GenBank database, they were identified as Lasiodiplodia theobromae, L. euphorbiaceicola and Neoscytalidium dimidiatum. ? 2023, Instituto de Investigaciones Agropecuarias, INIA. All rights reserved.
License
CC BY-NC
Rights
Authors
Publication Source
WOS