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Identification of sex-specific expression markers in the giant tiger shrimp (Penaeus monodon)
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Document Title
Identification of sex-specific expression markers in the giant tiger shrimp (Penaeus monodon)
Author
Khamnamtong B., Thumrungtanakit S., Klinbunga S., Aoki T., Hirono I., Menasveta P.
Name from Authors Collection
Affiliations
Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand; Program of Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Tokyo 108-8477, Japan; Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
Type
Article
Source Title
Journal of Biochemistry and Molecular Biology
ISSN
12258687
Year
2006
Volume
39
Issue
1
Page
37-45
Open Access
Bronze, Green
Publisher
The Biochemical Society of the Republic of Korea
DOI
10.5483/bmbrep.2006.39.1.037
Abstract
Bulked segregant analysis (BSA) and AFLP were used for isolation of genomic sex determination markers in Penaeus monodon. A total of 256 primer combinations were tested against 6-10 bulked genomic DNA of P. monodon. Five and one candidate female-and male-specific AFLP fragments were identified. Female-specific fragments were cloned and further characterized. SCAR markers derived from FE10M9520, FE10M10725.1, FE10M10725.2 and FE14M16340 provided the positive amplification product in both male and female P. monodon. Further analysis of these markers using SSCP and genome walk analysis indicated that they were not sex-linked. In addition, sex-specific (or differential) expression markers in ovaries and testes of P. monodon were analyzed by RAP-PCR (150 primer combinations). Twenty-one and fourteen RAP-PCR fragments specifically/ differentially expressed in ovaries and testes of P. monodon were successfully cloned and sequenced. Expression patterns of 25 transcripts were tested against the first stranded cDNA of ovaries and testes of 3-month-old and broodstock-sized P. monodon (N = 5 and N=7-10 for females and N = 4 and N = 5 - 7 for males, respectively). Five (FI-4, FI-44, FIII-4, FIII-39 and FIII-58) and two (M457-A01 and MII-51) derived RAP-PCR markers revealed female- and male-specific expression patterns in P. monodon. Surprisingly, MII-5 originally found in testes showed a higher expression level in ovaries than did testes of juvenile shrimps but a temporal female-specific pattern in P. monodon adults.
License
CC BY-NC
Rights
Author
Publication Source
Scopus