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Immune regulation, but not antibacterial activity, is a crucial function of hepcidins in resistance against pathogenic bacteria in nile tilapia (Oreochromis niloticus linn.)
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Metadata
Document Title
Immune regulation, but not antibacterial activity, is a crucial function of hepcidins in resistance against pathogenic bacteria in nile tilapia (Oreochromis niloticus linn.)
Author
Phan-Aram P., Mahasri G., Kayansamruaj P., Amparyup P., Srisapoome P.
Name from Authors Collection
Affiliations
Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Paholayothin Rd, Ladyao, Chatuchak, Bangkok, 10900, Thailand; Department of Fish Health Management and Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Campus C Mulyorejo, Surabaya, 60115, Indonesia; Marine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)Pathum Thani 12120, Thailand
Type
Article
Source Title
Biomolecules
ISSN
2218273X
Year
2020
Volume
10
Issue
8
Page
Jan-29
Open Access
Gold, Green
Publisher
MDPI AG
DOI
10.3390/biom10081132
Abstract
In this study, the functions of a recombinant propeptide (rProOn-Hep1) and the synthetic FITC-labelled mature peptides sMatOn-Hep1 and sMatOn-Hep2 were analyzed. Moreover, sMatOn-Hep1 and sMatOn-Hep2 were mildly detected in the lymphocytes of peripheral blood mononuclear cells (PBMCs) and strongly detected in head kidney macrophages. The in vitro binding and antibacterial activities of these peptides were slightly effective against several pathogenic bacteria. Immune regulation by sMatOn-Hep1 was also analyzed, and only sMatOn-Hep1 significantly enhanced the phagocytic index in vitro (p < 0.05). Interestingly, intraperitoneal injection of sMatOn-Hep1 (10 or 100 µg) significantly elevated the phagocytic activity, phagocytic index, and lysozyme activity and clearly decreased the iron ion levels in the livers of the treated fish (p < 0.05). Additionally, sMatOn-Hep1 enhanced the expression levels of CC and CXC chemokines, transferrin and both On-Hep genes in the liver, spleen and head kidney, for 1–96 h after injection, but did not properly protect the experimental fish from S. agalactiae infection after 7 days of treatment. However, the injection of S. agalactiae and On-Heps indicated that 100 µg of sMatOn-Hep1 was very effective, while 100 µg of rProOn-Hep1 and sMatOn-Hep2 demonstrated moderate protection. Therefore, On-Hep is a crucial iron-regulating molecule and a key immune regulator of disease resistance in Nile tilapia. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Funding Sponsor
Kasetsart University; National Research Council of Thailand; Kasetsart University Research and Development Institute
License
CC BY
Rights
Author
Publication Source
Scopus