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Impact of hydrolyzed collagen from defatted sea bass skin on proliferation and differentiation of preosteoblast MC3T3-E1 cells
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Document Title
Impact of hydrolyzed collagen from defatted sea bass skin on proliferation and differentiation of preosteoblast MC3T3-E1 cells
Author
Chotphruethipong L.,Binlateh T.,Hutamekalin P.,Aluko R.E.,Tepaamorndech S.,Zhang B.,Benjakul S.
Name from Authors Collection
Affiliations
International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; School of Geriatric Oral Health, Institute of Dentistry, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand; Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada; National Center of Genetic Engineering and Biotechnology Center (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Pathumthani12120, Thailand; College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China
Type
Article
Source Title
Foods
ISSN
23048158
Year
2021
Volume
10
Issue
7
Open Access
All Open Access, Gold, Green
Publisher
MDPI AG
DOI
10.3390/foods10071476
Abstract
Osteoporosis is a serious problem affecting health of the elderly. Drugs (bisphosphonates) applied for treatment are often accompanied by adverse side effects. Thus, fish byproduct-derived peptides, particularly hydrolyzed collagen (HC) from defatted sea bass skin, could be a safe source of anti-osteoporosis agents. This study aimed to examine the effects of HC on proliferation and differentiation of preosteoblast cells. HC prepared using papain before Alcalase hydrolysis was determined for molecular weight (MW) distribution. Thereafter, the resulting HC (50–800 µg/mL) was added to the cell. Proliferation, alkaline phosphatase activity (AP-A) and mineralization of cells were investigated. Moreover, the expression of runt-related transcription factor 2 (RUNX2) and the p-Akt/Akt pathway were also determined using Western blot. The results showed that HC had an MW < 3 kDa. HC (50–200 µg/mL) could promote cell proliferation. Nevertheless, HC at 100 µg/mL (HC-100) had enhanced AP-A and increased mineralization during the first 7 days of culture. Moreover, HC-treated cells had higher calcium depositions than the control (p < 0.05). Additionally, cells treated with HC-100 had higher levels of RUNX2 and p-Akt expressions than control (p < 0.05). Therefore, HC could be a promising functional ingredient to promote osteoblast proliferation and differentiation, which could enhance bone strength. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keyword
Bioactive peptides | bone | Differentiation | Hydrolyzed collagen | osteoblast | Sea bass
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency; Prince of Songkla University; National Research Council of Thailand
License
CC BY
Rights
Author
Publication Source
Scopus