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Improvement of the multi-performance biocharacteristics of cordycepin using BiloNiosome-core/chitosan-shell hybrid nanocarriers
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Document Title
Improvement of the multi-performance biocharacteristics of cordycepin using BiloNiosome-core/chitosan-shell hybrid nanocarriers
Author
Kengkittipat W., Kaewmalun S., Khongkow M., Iempridee T., Jantimaporn A., Bunwatcharaphansakun P., Yostawonkul J., Yata T., Phoolcharoen W., Namdee K.
Name from Authors Collection
Scopus Author ID
57194431058
Scopus Author ID
56026006800
Affiliations
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand; Research Unit for Plant-produced Pharmaceuticals, Chulalongkorn University, Bangkok, Thailand; National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency, 111 Thailand Science Park, Phahonyothin Rd., Khlong Luang, Pathumthani 12120, Thailand; Biochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Natural Products and Nanoparticles Research Unit, Chulalongkorn University, Bangkok, 10330, Thailand
Type
Article
Source Title
Colloids and Surfaces B: Biointerfaces
ISSN
09277765
Year
2021
Volume
197
Open Access
All Open Access, Hybrid Gold
Publisher
Elsevier B.V.
DOI
10.1016/j.colsurfb.2020.111369
Format
Abstract
Cordycepin, a derivative of the nucleotide adenosine, has displayed several pharmacological activities including enhanced apoptosis and cancer cells inhibition. However, oral administration of cordycepin has limited practical use due to its poor bioavailability in the intestine. Herein, we developed and demonstrated a hybrid nanocarrier system in the form of biloniosome-core/chitosan-shell hybrid nanocarriers (HNCs) in order to improve the bio-characteristics of cordycepin. In this study, HNCs were prepared by using a solvent (ethanol) injection method involving cordycepin as the biloniosome core and mucoadhesive chitosan biopolymer as a coating shell. Our results showed that the cordycepin-loaded HNCs were positively charged with enhanced mucoadhesive characteristics and highly stable in gastric fluid. The increased permeability of cordycepin-loaded HNCs compared with standard cordycepin was confirmed by in vitro intestinal permeation study across the human intestinal barrier. In addition, we demonstrated that the cordycepin-loaded HNCs are able to release their components in an active form resulting in enhanced anti-cancer activity in two-dimensional (2D) cell cultures as well as in three-dimensional (3D) multi-cellular spheroids of colon cancer cells. Further, quantitative real time PCR analysis of apoptotic gene expression revealed that cordycepin HNCs can induce apoptosis in cancer cells by negatively regulating the expression of B-cell lymphoma-extra large (BCL-XL). I Overall our results showed that the hybrid nanocarrier systems represent a promising strategy for improving the bio-characteristics of cordycepin which can be considered as a potential anti-cancer agent for colorectal cancer chemotherapy. © 2020 The Author(s)
Keyword
Colon cancer | Cordycepin | Hybrid nanocarrier | Oral administration
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Chulalongkorn University; National Science and Technology Development Agency; Thailand Research Fund; National Nanotechnology Center; Thailand Graduate Institute of Science and Technology; Thailand Science Research and Innovation
License
CC BY
Rights
Author
Publication Source
Scopus