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In experimental challenge with infectious clones of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV), MrNV alone can cause mortality in freshwater prawn (Macrobrachium rosenbergii)
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Document Title
In experimental challenge with infectious clones of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV), MrNV alone can cause mortality in freshwater prawn (Macrobrachium rosenbergii)
Author
Gangnonngiw W., Bunnontae M., Phiwsaiya K., Senapin S., Dhar A.K.
Name from Authors Collection
Scopus Author ID
6503848431
Affiliations
Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, 10400, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Klong 1, Klong Luang, Pratum Thani 12120, Thailand; Aquaculture Pathology Laboratory, School of Animal and Comparative Biomedical Sciences, University of Arizona, Building 90, 1117 E. Lowell St., Tucson, AZ 85718, United States
Type
Article
Source Title
Virology
ISSN
00426822
Year
2020
Volume
540
Page
30-37
Open Access
Bronze
Publisher
Academic Press Inc.
DOI
10.1016/j.virol.2019.11.004
Abstract
To overcome the lack of immortal shrimp cell lines for shrimp viral research, we constructed and tested DNA infectious clones of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) often found together in freshwater prawn (M. rosenbergii) exhibiting white tail disease (WTD). Full-length cDNAs of MrNV and XSV genomic RNA were individually inserted into the baculovirus pFastBacDUAL shuttle vector. Individual Sf9 (insect cell line) transfection resulted in production of RNA (RT-PCR) and capsid proteins (immunofluorescence) for both viruses. Presence of respective virions was confirmed by density gradient purification followed by RT-PCR and transmission electron microscopy. Infectivity was by tested in immersion-challenge tests with M. rosenbergii post-larvae (PL) using both semi-purified viruses, individually or combined, and confirmed by histological analysis (morphology and immunofluorescence) and quantitative RT-PCR. Mortality accompanied by WTD lesions occurred with MrNV alone or in combination with XSV but not with XSV alone, despite its replication. © 2019 Elsevier Inc.
Keyword
Freshwater prawn | Infectious clone | M. rosenbergii | MrNV | White tail disease | XSV
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Srinakharinwirot University; Mahidol University
License
CC BY or CC BY-NC-ND
Rights
Elsevier B.V.
Publication Source
Scopus