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In vitro analysis of the anticancer activity ofLysinibacillus sphaericusbinary toxin in human cancer cell lines
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Document Title
In vitro analysis of the anticancer activity ofLysinibacillus sphaericusbinary toxin in human cancer cell lines
Author
Chankamngoen W, Janvilisri T, Promdonkoy B, Boonserm P
Name from Authors Collection
Affiliations
Mahidol University; Mahidol University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
3 BIOTECH
ISSN
2190-572X
Year
2020
Volume
10
Open Access
Green Published
Publisher
SPRINGER HEIDELBERG
DOI
10.1007/s13205-020-02361-8
Format
Abstract
Binary or Bin toxin produced byLysinibacillus sphaericusis composed of BinA (42 kDa) and BinB (51 kDa) subunits. These work together to exert maximal toxicity against mosquito larvae via pore formation and induction of apoptosis. The C-terminal domains in both subunits are homologous to those of aerolysin-type beta pore-forming toxins, including parasporin-2 (PS2). The latter is one of theBacillus thuringiensistoxins that exhibits specific cytotoxicity against human cancer cells. The present study investigates the possible anticancer activity of Bin toxin using PS2 as a control. We demonstrate that treatment with a high concentration of trypsin-activated Bin inhibits cell proliferation in human cancer cells A549, Caco-2, HepG2, HK-1 and KKU-M055. In the most susceptible cells, HK-1, Bin toxin exposure led to morphological alterations, decreased migration, decreased adhesion activity and apoptosis induction. Although these effects necessitated high concentrations, they suggest that Bin toxin may be optimized as a novel potential cancer-therapeutic agent.
Funding Sponsor
Synchrotron Light Research Institute (Public Organization) [BRG5980016]; Thailand Research Fund, Mahidol University; Thailand Graduated Institute of Science and Technology (TGIST)
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Publication Source
WOS