• Influence of Lithium-and Zinc-Containing Bioactive Glasses on Pulpal Regeneration

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Document Title

Influence of Lithium-and Zinc-Containing Bioactive Glasses on Pulpal Regeneration

Author

Tran A.T.L. Sukajintanakarn C. Senawongse P. Sritanaudomchai H. Ruangsawasdi N. Lapthanasupkul P. Kitkumthorn N. Monmaturapoj N. Khamsut C. Naruphontjirakul P. Pongprueksa P.

Affiliations

Dental Biomaterials Science Program Faculty of Dentistry Mahidol University Bangkok Thailand; Department of Conservative Dentistry and Prosthodontics Faculty of Dentistry Srinakharinwirot University Bangkok Thailand; Department of Operative Dentistry and Endodontics Faculty of Dentistry Mahidol University Bangkok Thailand; Department of Oral Biology Faculty of Dentistry Mahidol University Bangkok Thailand; Department of Pharmacology Faculty of Dentistry Mahidol University Bangkok Thailand; Department of Oral and Maxillofacial Pathology Faculty of Dentistry Mahidol University Bangkok Thailand; Assistive Technology and Medical Devices Research Center National Science and Technology Development Agency Pathum Thani Thailand; Biological Engineering Program Faculty of Engineering King Mongkut's University of Technology Thonburi Bangkok Thailand

Type

Article

Source Title

European Journal of Dentistry

ISSN

13057456

Year

2023

Volume

17

Issue

4

Page

1120-1128

Open Access

All Open Access Hybrid Gold Green

Publisher

Georg Thieme Verlag

DOI

10.1055/s-0042-1758789

Abstract

Objective To evaluate the potential of modified bioactive glasses containing lithium and zinc as pulp capping materials by investigating the odontogenic differentiation and mineralization response in the tooth culture model. Materials and Methods Lithium-and zinc-containing bioactive glasses (45S5.1Li 45S5.5Li 45S5.1Zn 45S5.5Zn 45S5.1Zn sol-gel and 45S5.5Zn sol-gel) fibrinogen-Thrombin and biodentine were prepared to assess Axin2 gene expression at 0 30 minutes 1 hour 12 hours and 1 day and DSPP gene expression at 0 3 7 and 14 days in stem cells from human exfoliated deciduous teeth (SHEDs) using qRT-PCR. The experimental bioactive glasses incorporated with fibrinogen-Thrombin and biodentine were placed on the pulpal tissue in the tooth culture model. Histology and immunohistochemistry were analyzed at 2 weeks and 4 weeks. Results Axin2 gene expression for all experimental groups was significantly higher than the control at 12 hours. The DSPP gene expression for all experimental groups was significantly higher than the control at 14 days. The presence of mineralization foci was significantly higher at 4 weeks for the modified bioactive glasses 45S5.5Zn 45S5.1Zn sol-gel and 45S5.5Zn sol-gel as well as Biodentine compared with the fibrinogen-Thrombin control. Conclusion Lithium-and zinc-containing bioactive glasses increased Axin2 and DSPP gene expression in SHEDs and can potentially enhance pulp mineralization and regeneration. Zinc-containing bioactive glasses are a promising candidate to be used as pulp capping materials. ? 2023 Georg Thieme Verlag. All rights reserved.

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