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Integrated Proteomic and Metabolomic Analysis of Cassava cv. Kasetsart 50 Infected with Sri Lankan Cassava Mosaic Virus
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Document Title
Integrated Proteomic and Metabolomic Analysis of Cassava cv. Kasetsart 50 Infected with Sri Lankan Cassava Mosaic Virus
Author
Siriwan W., Vannatim N., Chaowongdee S., Roytrakul S., Charoenlappanit S., Pongpamorn P., Paemanee A., Malichan S.
Affiliations
Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand; Center of Excellence on Agricultural Biotechnology (AG-BIO/MHESI), Bangkok, 10900, Thailand; Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand; National Center for Genetic and Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; National Omics Center (NOC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand
Type
Article
Source Title
Agronomy
ISSN
20734395
Year
2023
Volume
13
Issue
3
Page
-
Open Access
All Open Access, Gold
Publisher
MDPI
DOI
10.3390/agronomy13030945
Format
Abstract
Cassava mosaic disease (CMD) is a major disease affecting cassava production in Southeast Asia. This study aimed to perform an integrated proteomics and metabolomics analysis of cassava cv. Kasetsart 50 infected with Sri Lankan cassava mosaic virus (SLCMV). Proteomics analyses revealed that 359 proteins were enriched in the plant–pathogen interaction, plant hormone signal transduction, and MAPK signaling pathways. A total of 79 compounds were identified by metabolomics analysis of the healthy and SLCMV-infected cassava plants. Integrated omics analysis revealed that 9 proteins and 5 metabolites were enriched in 11 KEGG pathways. The metabolic pathways, plant hormone signal transduction, and plant–pathogen interaction pathway terms were specifically investigated. The findings revealed that caffeic acid and chlorogenic acid were associated with the plant–pathogen interaction pathway, histidine (HK3) was involved in plant hormone signal transduction, while citric acid and D-serine were associated with the metabolic pathways. KEGG functional enrichment analysis revealed that plant–pathogen interaction, plant hormone signal transduction, and metabolic pathways were linked via the enriched protein (protein phosphatase 2C) and metabolites (cyclic nucleotide-binding (AT2G20050) and D-serine). The available information and resources for proteomics and metabolomics analyses of cassava can elucidate the mechanism of disease resistance and aid in cassava crop improvement programs. © 2023 by the authors.
Funding Sponsor
Kasetsart University Research and Development Institute
Publication Source
WOS