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Invariant NKT Cell Response to Dengue Virus Infection in Human
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Metadata
Document Title
Invariant NKT Cell Response to Dengue Virus Infection in Human
Author
Matangkasombut P.,Chan-in W.,Opasawaschai A.,Pongchaikul P.,Tangthawornchaikul N.,Vasanawathana S.,Limpitikul W.,Malasit P.,Duangchinda T.,Screaton G.,Mongkolsapaya J.
Name from Authors Collection
Scopus Author ID
6507262736
Affiliations
Department of Microbiology, Mahidol University, Bangkok, Thailand; Systems Biology of Diseases Research Unit, Mahidol University, Bangkok, Thailand; Center of Emerging and Neglected Infectious Diseases, Mahidol University, Bangkok, Thailand; Medical Biotechnology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, Thailand; Department of Pediatrics, Khon Kaen Hospital, Khon Kaen, Thailand; Department of Pediatrics, Songkhla hospital, Songkhla, Thailand; Dengue Hemorrhagic Fever Research Unit, Office for Research and Development, Siriraj Hospital, Mahidol University, Bangkok, Thailand; Division of Immunology and Inflammation, Department of Medicine, Hammersmith campus, Imperial College London, London, United Kingdom
Type
Article
Source Title
PLoS Neglected Tropical Diseases
ISSN
19352727
Year
2014
Volume
8
Issue
6
Open Access
All Open Access, Gold, Green
Publisher
Public Library of Science
DOI
10.1371/journal.pntd.0002955
Abstract
Background:Dengue viral infection is a global health threat without vaccine or specific treatment. The clinical outcome varies from asymptomatic, mild dengue fever (DF) to severe dengue hemorrhagic fever (DHF). While adaptive immune responses were found to be detrimental in the dengue pathogenesis, the roles of earlier innate events remain largely uninvestigated. Invariant natural killer T (iNKT) cells represent innate-like T cells that could dictate subsequent adaptive response but their role in human dengue virus infection is not known. We hypothesized that iNKT cells play a role in human dengue infection.Methods:Blood samples from a well-characterized cohort of children with DF, DHF, in comparison to non-dengue febrile illness (OFI) and healthy controls at various time points were studied. iNKT cells activation were analyzed by the expression of CD69 by flow cytometry. Their cytokine production was then analyzed after α-GalCer stimulation. Further, the CD1d expression on monocytes, and CD69 expression on conventional T cells were measured.Results:iNKT cells were activated during acute dengue infection. The level of iNKT cell activation associates with the disease severity. Furthermore, these iNKT cells had altered functional response to subsequent ex vivo stimulation with α-GalCer. Moreover, during acute dengue infection, monocytic CD1d expression was also upregulated and conventional T cells also became activated.Conclusion:iNKT cells might play an early and critical role in the pathogenesis of severe dengue viral infection in human. Targeting iNKT cells and CD1d serve as a potential therapeutic strategy for severe dengue infection in the future. © 2014 Matangkasombut et al.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Author
Publication Source
Scopus