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Invertebrate Hematopoiesis: An Anterior Proliferation Center As a Link Between the Hematopoietic Tissue and the Brain
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Document Title
Invertebrate Hematopoiesis: An Anterior Proliferation Center As a Link Between the Hematopoietic Tissue and the Brain
Author
Noonin C, Lin XH, Jiravanichpaisal P, Soderhall K, Soderhall I
Name from Authors Collection
Affiliations
Uppsala University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
STEM CELLS AND DEVELOPMENT
ISSN
1547-3287
Year
2012
Volume
21
Open Access
Green Submitted
Publisher
MARY ANN LIEBERT, INC
DOI
10.1089/scd.2012.0077
Format
Abstract
During evolution, the innate and adaptive immune systems were developed to protect organisms from non-self substances. The innate immune system is phylogenetically more ancient and is present in most multicellular organisms, whereas adaptive responses are restricted to vertebrates. Arthropods lack the blood cells of the lymphoid lineage and oxygen-carrying erythrocytes, making them suitable model animals for studying the regulation of the blood cells of the innate immune system. Many crustaceans have a long life span and need to continuously synthesize blood cells, in contrast to many insects. The hematopoietic tissue (HPT) of Pacifastacus leniusculus provides a simple model for studying hematopoiesis, because the tissue can be isolated, and the proliferation of stem cells and their differentiation can be studied both in vivo and in vitro. Here, we demonstrate new findings of a physical link between the HPT and the brain. Actively proliferating cells were localized to an anterior proliferation center (APC) in the anterior part of the tissue near the area linking the HPT to the brain, whereas more differentiated cells were detected in the posterior part. The central areas of HPT expand in response to lipopolysaccharide-induced blood loss. Cells isolated from the APC divide rapidly and form cell clusters in vitro; conversely, the cells from the remaining HPT form monolayers, and they can be induced to differentiate in vitro. Our findings offer an opportunity to learn more about invertebrate hematopoiesis and its connection to the central nervous system, thereby obtaining new information about the evolution of different blood and nerve cell lineages.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Swedish Research Council [VR 621-2009-5715, VR 319-2010-6250, VR621-2011-4797]
Rights
Publisher
Publication Source
WOS