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Investigation on the physical properties and biocompatibility of zirconia-alumina-silicate@diopside composite materials and its in vivo toxicity study in embryonic zebrafish
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Document Title
Investigation on the physical properties and biocompatibility of zirconia-alumina-silicate@diopside composite materials and its in vivo toxicity study in embryonic zebrafish
Author
Rittidach T. Sillapaprayoon S. Chantho V. Pimtong W. Charoenphandhu N. Thongbunchoo J. Krishnamra N. Bootchanont A. Porjai P. Pon-On W.
Affiliations
Department of Physics Faculty of Science Kasetsart University Bangkok 10900 Thailand; Nano Environmental and Health Safety Research Team National Nanotechnology Center (NANOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani 12120 Thailand; Center of Calcium and Bone Research Faculty of Science Mahidol University Bangkok 10400 Thailand; Department of Physiology Faculty of Science Mahidol University Bangkok 10400 Thailand; Institute of Molecular Biosciences Mahidol University Nakhon Pathom 73170 Thailand; The Academy of Science The Royal Society of Thail Dusit Bangkok 10300 Thailand; Division of Physics Faculty of Science and Technology Rajamangala University of Technology Thanyaburi Pathum Thani 12120 Thailand
Type
Article
Source Title
RSC Advances
ISSN
20462069
Year
2023
Volume
13
Issue
44
Page
30575-30585
Open Access
All Open Access Gold Green
Publisher
Royal Society of Chemistry
DOI
10.1039/d3ra04555b
Abstract
Bioceramic materials have a wide range of applications in the biomedical field such as in the repair of bone defects and dental surgery. Silicate-based bioceramics have attracted biomedical researchers interest due to their bioactivity and biodegradability. In this study extended the scope of ZAS utilization in bone tissue engineering by introducing calcium-magnesium-silicate (diopside CMS) as an interface material aim to develop a machinable bioceramic composite (ZASCMS) by the sol-gel method. The physicochemical characterization in vitro biological properties and in vivo zebrafish cytotoxicity study of ZAS-based composites as a function of CMS contents 0 25 50 75 and 100 wt% were performed. Results showed that the as-prepared ZASCMS possessed porous architecture with well-interconnected pore structure. Results also revealed that the mechanical properties of ZASCMS composite materials were gradually improved with increasing CMS contents. The ZASCMS composites with more than 50 wt% CMS had the highest compressive strength and modulus of 6.78 ? 0.62 MPa and 340.10 ? 16.81 MPa respectively. Regarding in vitro bioactivities the composite scaffolds were found to stimulate osteoblast-like UMR-106 cell adhesion growth and proliferation. The antibacterial activity of the ZASCMS composite scaffolds was tested against Staphylococcus epidermidis (S. epidermidis) and Escherichia coli (E. coli) also exhibited an antibacterial property. Furthermore the in vivo studies using embryonic zebrafish were exposed to as-prepared particles (0-500 ?g mL?1) and showed that the synthesized ZAS CMS and ZASCMS composite particles were non-toxic based on the evaluation of survivability hatching rate and embryonic morphology. In conclusions our results indicated that the synthesized composite exhibited their biological properties and antibacterial activity which could well be a promising material with high potential to be applied in orthopaedic and dental tissue engineering. ? 2023 The Royal Society of Chemistry.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY-NC
Rights
The Royal Society of Chemistry
Publication Source
WOS