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Isolation and characterization of proline/betaine transporter gene from oil palm
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Document Title
Isolation and characterization of proline/betaine transporter gene from oil palm
Author
Yamada N, Cha-Um S, Kageyama H, Promden W, Tanaka Y, Kirdmanee C, Takabe T
Name from Authors Collection
Affiliations
Meijo University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Meijo University; Meijo University
Type
Article
Source Title
TREE PHYSIOLOGY
ISSN
0829-318X
Year
2011
Volume
31
Open Access
Bronze
Publisher
OXFORD UNIV PRESS
DOI
10.1093/treephys/tpr017
Format
Abstract
Oil production from oil palm is adversely affected by drought and salt. Under drought and salt stress, proline content increases in oil palm; the mechanism for this is unknown. Here, an 8319-nucleotide sequence including cDNA, genomic DNA and the promoter region of proline transporter gene from oil palm Elaeis guineensis was determined. The transporter gene exhibited high similarity to Bet/ProT genes from several plants, but the highest homology was found with rice ProT1. The exon-intron structure of genomic DNA was unique, and numerous stress-response cis-elements were found in the promoter region. Expression of cDNA EgProT1 in Escherichia coli mutant exhibited uptake activities for glycinebetaine and choline as well as proline. Under salt-stressed conditions, exogenously applied glycinebetaine was taken up into the root more rapidly than the control. These data indicate that oil palm has a unique Pro/T1 gene. Nucleotide sequence data for the cDNA and genomic DNA of proline transporter gene from Elaeis guineensis are available in the DDJB database under accession numbers AB597035 and AB597036, respectively.
Keyword
betaine transport | Elaeis guineensis | Oil palm | proline transporter | Salt stress
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Ministry of Education, Science and Culture of Japan; Salt Science Research Foundation; High-Tech Research Center of Meijo University
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Copyright
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Publication Source
WOS