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Isoprene-Degrading Bacteria from Soils Associated with Tropical Economic Crops and Framework Forest Trees
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Document Title
Isoprene-Degrading Bacteria from Soils Associated with Tropical Economic Crops and Framework Forest Trees
Author
Uttarotai T, McKew BA, Benyahia F, Murrell JC, Mhuantong W, Wangkarn S, Chitov T, Bovonsombut S, McGenity TJ
Name from Authors Collection
Affiliations
Chiang Mai University; Chiang Mai University; University of Essex; University of East Anglia; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Chiang Mai University; Chiang Mai University
Type
Article
Source Title
MICROORGANISMS
Year
2021
Volume
9
Issue
5
Open Access
Green Published, Green Accepted, gold
Publisher
MDPI
DOI
10.3390/microorganisms9051024
Format
Abstract
Isoprene, a volatile hydrocarbon emitted largely by plants, plays an important role in regulating the climate in diverse ways, such as reacting with free radicals in the atmosphere to produce greenhouse gases and pollutants. Isoprene is both deposited and formed in soil, where it can be consumed by some soil microbes, although much remains to be understood about isoprene consumption in tropical soils. In this study, isoprene-degrading bacteria from soils associated with tropical plants were investigated by cultivation and cultivation-independent approaches. Soil samples were taken from beneath selected framework forest trees and economic crops at different seasons, and isoprene degradation in soil microcosms was measured after 96 h of incubation. Isoprene losses were 4-31% and 15-52% in soils subjected to a lower (7.2 x 10(5) ppbv) and a higher (7.2 x 10(6) ppbv) concentration of isoprene, respectively. Sequencing of 16S rRNA genes revealed that bacterial communities in soil varied significantly across plant categories (framework trees versus economic crops) and the presence of isoprene, but not with isoprene concentration or season. Eight isoprene-degrading bacterial strains were isolated from the soils and, among these, four belong to the genera Ochrobactrum, Friedmanniella, Isoptericola and Cellulosimicrobium, which have not been previously shown to degrade isoprene.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Science Achievement Scholarship of Thailand, Biodiversitybased Economy Development Office (Public Organization) (BEDO; Natural Environment Research Council (NERC) [NE/J009555/1]; European Research Council (ERC) [IsoMet 694578]; Chiang Mai University, Thailand; University of Essex, UK
License
CC-BY
Rights
Authors
Publication Source
WOS