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Jiangella endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans
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Document Title
Jiangella endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans
Author
Niemhom N, Chutrakul C, Suriyachadkun C, Tadtong S, Thawai C
Name from Authors Collection
Affiliations
King Mongkuts Institute of Technology Ladkrabang; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Srinakharinwirot University; King Mongkuts Institute of Technology Ladkrabang
Type
Article
Source Title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN
1466-5026
Year
2019
Volume
69
Issue
2
Page
454-459
Open Access
Bronze
Publisher
MICROBIOLOGY SOC
DOI
10.1099/ijsem.0.003175
Format
Abstract
An endophytic actinobacterium, designated strain KE2-3(T) , was isolated from surface-sterilised rhizome of Kaempferia elegans. The polyphasic approach was used for evaluating the taxonomic position of this strain. The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of LL-diaminopimelic acid in the cell peptidoglycan, MK-9(H-4) as the major menaquinone, iso-C-16:0, anteiso-C-15:0, iso-C-14:0 and iso-C-15:0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes. Based on 16S rRNA gene sequence analysis, strain KE2-3(T) was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357(T) (99.3 %) followed by Jiangella alba DSM 45237(T )(99.2 %), Jiangella alkaliphiliaDSM 45079(T) (99.0 %), Jiangella gansuensis DSM 44835(T) (98.8 %) and Jiangella mangrovi 3SM4-07(T) (98.6 %). However, the draft genome sequence of strain KE2-3(T) exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96 % species circumscription threshold. The DNA G+C content of genomic DNA was 72.3 mol%. With the differences of physiological, biochemical and genotypic data, strain KE2-3(T) could be discriminated from its closest neighbour. Thus, strain KE2-3(T) should be recognised as a novel species of genus Jiangella, for which the name Jiangella endophytica sp. nov. is proposed. The type strain is KE2-3(T)(=BCC 66359(T)=NBRC 110004(T)).
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
King Mongkut's Institute of Technology Ladkrabang Research Fund (KReF) [KREF045905]; Thailand Graduate Institute of Science and Technology (TGIST), National Science and Technology Development Agency (NSTDA) [TG-22-22-55-004D]
License
Copyright
Rights
IUMS
Publication Source
WOS