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Kallikrein-11, in association with coiled-coil domain containing 25, as a potential prognostic marker for cholangiocarcinoma with lymph node metastasis
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Metadata
Document Title
Kallikrein-11, in association with coiled-coil domain containing 25, as a potential prognostic marker for cholangiocarcinoma with lymph node metastasis
Author
Siriphak S., Chanakankun R., Proungvitaya T., Roytrakul S., Tummanatsakun D., Seubwai W., Wongwattanakul M., Proungvitaya S.
Name from Authors Collection
Affiliations
Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, KhonKaen University, Khon Kaen, 40002, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyPathum Thani 12120, Thailand; Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Forensic Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
Type
Article
Source Title
Molecules
ISSN
14203049
Year
2021
Volume
26
Issue
11
Open Access
Gold, Green
Publisher
MDPI AG
DOI
10.3390/molecules26113105
Abstract
Cholangiocarcinoma (CCA) is a malignancy arising from cholangiocytes. Currently, the treatment and prognosis for CCA are mostly poor. Recently, we have reported that coiled-coil domain containing 25 (CCDC25) protein level in the sera may be a diagnostic marker for CCA. Subsequently, we identified three binding proteins of CCDC25 and found that kallikrein-11 (KLK11) expression was highest among those binding proteins. In this study, we investigated CCDC25 and KLK11 expression in CCA and adjacent normal tissues (n = 18) using immunohistochemistry. The results demonstrated that the expressions of CCDC25 and KLK11 in CCA tissues were both significantly higher than the adjacent tissues (p < 0.001 and p = 0.001, respectively). Then, using GEPIA bioinformatics analysis, KLK11 mRNA was significantly overexpressed in CCA tumor tissues compared with normal tissues (p < 0.05). Moreover, CCDC25 expression was positively correlated with KLK11 expression in CCA with lymph node metastasis (p = 0.028, r = 0.593). An analysis for the interaction of KLK11 with CCDC25 and other proteins, using STRING version 11.0, revealed that CCDC25 and KLK11 correlated with metastasis-related proteins. In addition, Kaplan-Meier survival curve analysis revealed that a high expression of KLK11 was associated with the poor prognosis of CCA. In conclusion, KLK11 is, as a binding protein for CCDC25, possibly involved in the metastatic process of CCA. KLK11 may be used as a prognostic marker for CCA. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keyword
CCDC25 | Cholangiocarcinoma | Immunohistochemistry | KLK11 | Prognostic marker
Funding Sponsor
Khon Kaen University; Khon Kean University
License
CC BY
Rights
Author
Publication Source
Scopus