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Kutzneria buriramensis sp nov., isolated from soil, and emended description of the genus Kutzneria
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Document Title
Kutzneria buriramensis sp nov., isolated from soil, and emended description of the genus Kutzneria
Author
Suriyachadkun C, Ngaemthao W, Chunhametha S, Tamura T, Sanglier JJ
Name from Authors Collection
Affiliations
National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Novartis
Type
Article
Source Title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN
1466-5026
Year
2013
Volume
63
Page
47-52
Open Access
Bronze
Publisher
MICROBIOLOGY SOC
DOI
10.1099/ijs.0.036533-0
Format
Abstract
A Gram-staining-positive, filamentous bacterium, which developed large globose sporangia at the ends of long sporangiophores on aerial mycelium, was isolated from dry soil collected in a deciduous forest in Thailand. The cell-wall peptidoglycan of the novel bacterium, which was designated strain A-T 1846(T), contained meso-diaminopimelic acid and the whole-cell sugars comprised rhamnose, ribose, mannose, glucose and galactose. The predominant menaquinone was MK-9(H-4). Mycolic acids were not detected. The identified phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol. The predominant cellular fatty acids were iso-C-16:0, iso-C-16:0 2-OH and C-16:0 10-methyl. The G+C content of the genomic DNA was 72 mol%. The phenotypic and chemotaxonomic characteristics of the novel strain resembled those of established members of the genus Kutzneria. Phylogenetic analyses based on 16S rRNA gene sequences also indicated that this strain belonged to the genus Kutzneria but as a representative of a putative novel species. In hybridization experiments, the DNA-DNA relatedness values recorded between the novel strain and established members of the genus Kutzneria all fell well below 70%. Based on the phenotypic, chemotaxonomic and genotypic evidence and the results of the DNA-DNA hybridizations, strain A-T 1846(T) represents a novel species in the genus Kutzneria, for which the name Kutzneria buriramensis sp. nov. is proposed. The type strain is A-T 1846(T) (=BCC 29373(T) =NBRC 107931(T)). An emended description of the genus Kutzneria is provided.
Funding Sponsor
Bioresources Research Network, Bangkok, Thailand
Publication Source
WOS