• Magnetic Graphene Oxide Nanocomposites for Selective miRNA Separation and Recovery

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Document Title

Magnetic Graphene Oxide Nanocomposites for Selective miRNA Separation and Recovery

Author

Uten S. Boonbanjong P. Prueksathaporn Y. Treerattrakoon K. Sathirapongsasuti N. Chanlek N. Pinitsoontorn S. Luksirikul P. Japrung D.

Affiliations

Department of Chemistry Faculty of Science Kasetsart University Bangkok 10900 Thailand; Program in Translational Medicine Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok 10400 Thailand; National Nanotechnology Center (NANOTEC) National Science and Technology Development Agency (NSTDA) Thailand Science Park Pathumthani 12120 Thailand; Department of Pure and Applied Chemistry Technology and Innovation Centre University of Strathclyde Glasgow G1 1RD United Kingdom; Synchrotron Light Research Institute (Public Organization) 111 University Avenue Nakhon Ratchasrima 30000 Thailand; Institute of Nanomaterials Research and Innovation for Energy (IN-RIE) Khon Kaen University Khon Kaen 40002 Thailand; Research Network NANOTEC-KU on Nanocatalysts and Nanomaterials for Sustainable Energy and Environment Kasetsart University Bangkok 10900 Thailand

Type

Article

Source Title

ACS Omega

ISSN

24701343

Year

2024

Volume

9

Issue

2

Page

2263-2271

Open Access

All Open Access Gold Green

Publisher

American Chemical Society

DOI

10.1021/acsomega.3c05919

Abstract

In this study we developed magnetic graphene oxide composites by chemically attaching Fe3O4 nanoparticles to graphene oxide nanosheets. Characterization techniques including Fourier transform infrared spectroscopy (FTIR) X-ray powder diffraction (XRD) Raman spectroscopy thermogravimetric analysis (TGA) X-ray photoelectron spectroscopy (XPS) atomic force microscopy (AFM) and transmission electron microscopy (TEM) confirmed the successful synthesis of Fe3O4@GO composites with desirable properties. The resulting composites exhibited superparamagnetic behavior solubility and compatibility for efficient miRNA separation. Using miR-29a as a model we demonstrated the effective binding of miR-29a to the magnetic graphene oxide (GO) composites at an optimal concentration of 1.5 mg/mL followed by a simple separation using magnetic forces. Additionally the addition of 5.0 M urea enhanced the miRNA recovery. These findings highlight the potential use of our magnetic graphene oxide composites for the efficient separation and recovery of miR-29a suggesting their broad applicability in various miRNA-based studies. Further exploration can focus on investigating endogenous miRNAs with aberrant expression patterns contributing to the advancements in precision medicine. ? 2024 The Authors. Published by American Chemical Society

Industrial Classification

N/A

Knowledge Taxonomy Level 1

N/A

Knowledge Taxonomy Level 2

N/A

Knowledge Taxonomy Level 3

N/A

License

CC BY-NC-ND

Rights

Authors

Publication Source

WOS

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