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Metabolic regulation of sugar assimilation for lipid production in aspergillus oryzae bcc7051 through comparative transcriptome perspective
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Document Title
Metabolic regulation of sugar assimilation for lipid production in aspergillus oryzae bcc7051 through comparative transcriptome perspective
Author
Vorapreeda T., Khongto B., Thammarongtham C., Srisuk T., Laoteng K.
Name from Authors Collection
Scopus Author ID
54880593800
Affiliations
Biochemical Engineering and Systems Biology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), King Mongkut’s University of Technology Thonburi, Bangkok, 10150, Thailand; Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathum Thani12120, Thailand; Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, 10150, Thailand
Type
Article
Source Title
Biology
ISSN
20797737
Year
2021
Volume
10
Issue
9
Open Access
All Open Access, Gold, Green
Publisher
MDPI
DOI
10.3390/biology10090885
Format
Abstract
Microbial lipid production with cost effectiveness is a prerequisite for the oleochemical sector. In this work, genome-wide transcriptional responses on the utilization of xylose and glucose in oleaginous Aspergillus oryzae were studied with relation to growth and lipid phenotypic traits. Comparative analysis of the active growth (t1) and lipid-accumulating (t2) stages showed that the C5 cultures efficiently consumed carbon sources for biomass and lipid production comparable to the C6 cultures. By pairwise comparison, 599 and 917 differentially expressed genes (DEGs) were identified in the t1 and t2 groups, respectively, in which the consensus DEGs were categorized into polysaccharide-degrading enzymes, membrane transports, and cellular processes. A discrimination in transcriptional responses of DEGs set was also found in various metabolic genes, mostly in car-bohydrate, amino acid, lipid, cofactors, and vitamin metabolisms. Although central carbohydrate metabolism was shared among the C5 and C6 cultures, the metabolic functions in acetyl-CoA and NADPH generation, and biosynthesis of terpenoid backbone, fatty acid, sterol, and amino acids were allocated for leveraging biomass and lipid production through at least transcriptional control. This study revealed robust metabolic networks in the oleaginicity of A. oryzae governing glucose/xylose flux toward lipid biosynthesis that provides meaningful hints for further process developments of microbial lipid production using cellulosic sugar feedstocks. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keyword
Aspergillus oryzae | Carbon source | Lipid production | Oleaginous fungi | Transcriptome
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency
License
N/A
Rights
N/A
Publication Source
Scopus