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Metabolic responses of carotenoid and cordycepin biosynthetic pathways in cordyceps militaris under light-programming exposure through genome-wide transcriptional analysis
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Metadata
Document Title
Metabolic responses of carotenoid and cordycepin biosynthetic pathways in cordyceps militaris under light-programming exposure through genome-wide transcriptional analysis
Author
Thananusak R., Laoteng K., Raethong N., Zhang Y., Vongsangnak W.
Name from Authors Collection
Affiliations
Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; Industrial Bioprocess Technology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)Pathum Thani 12120, Thailand; Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, 225009, China; Omics Center for Agriculture, Bioresources, Food, and Health, Kasetsart University (OmiKU), Bangkok, 10900, Thailand
Type
Article
Source Title
Biology
ISSN
20797737
Year
2020
Volume
9
Issue
9
Page
Jan-14
Open Access
All Open Access, Gold, Green
Publisher
MDPI AG
DOI
10.3390/biology9090242
Abstract
Cordyceps militaris is currently exploited for commercial production of specialty products as its biomass constituents are enriched in bioactive compounds, such as cordycepin. The rational process development is important for economically feasible production of high quality bioproducts. Light is an abiotic factor affecting the cultivation process of this entomopathogenic fungus, particularly in its carotenoid formation. To uncover the cell response to light exposure, this study aimed to systematically investigate the metabolic responses of C. militaris strain TBRC6039 using integrative genome-wide transcriptome and genome-scale metabolic network (GSMN)-driven analysis. The genome-wide transcriptome analysis showed 8747 expressed genes in the glucose and sucrose cultures grown under light-programming and dark conditions. Of them, 689 differentially expressed genes were significant in response to the light-programming exposure. Through integration with the GSMN-driven analysis using the improved network (iRT1467), the reporter metabolites, e.g., adenosine-5′-monophosphate (AMP) and 2-oxoglutarate, were identified when cultivated under the carotenoid-producing condition controlled by light-programming exposure, linking to up-regulations of the metabolic genes involved in glyoxalase system, as well as cordycepin and carotenoid biosynthesis. These results indicated that C. militaris had a metabolic control in acclimatization to light exposure through transcriptional co-regulation, which supported the cell growth and cordycepin production in addition to the accumulation of carotenoid as a photo-protective bio-pigment. This study provides a perspective in manipulating the metabolic fluxes towards the target metabolites through either genetic or physiological approaches. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Funding Sponsor
Thailand Research Fund; Kasetsart University; School of Aerospace Science and Technology
License
CC BY
Rights
Author
Publication Source
Scopus