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Metal Oxide Nanostructures Enhanced Microfluidic Platform for Efficient and Sensitive Immunofluorescence Detection of Dengue Virus
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Document Title
Metal Oxide Nanostructures Enhanced Microfluidic Platform for Efficient and Sensitive Immunofluorescence Detection of Dengue Virus
Author
Pormrungruang P. Phanthanawiboon S. Jessadaluk S. Larpthavee P. Thaosing J. Rangkasikorn A. Kayunkid N. Waiwijit U. Horprathum M. Klamchuen A. Pruksamas T. Puttikhunt C. Yasui T. Djamal M. Rahong S. Nukeaw J.
Affiliations
College of Materials Innovation and Technology King Mongkut抯 Institute of Technology Ladkrabang Chalongkrung Rd. Ladkrabang Bangkok 10520 Thailand; Department of Microbiology Faculty of Medicine Khon Kaen University Khon Kaen 40002 Thailand; National Electronics and Computer Technology Center National Science and Development Agency Pathumtani 12120 Thailand; National Nanotechnology Center National Science and Development Agency Pathumtani 12120 Thailand; National Center for Genetic and Engineering and Biotechnology (BIOTEC) National Science and Development Agency Pathumtani 12120 Thailand; Department of Life Science and Technology Tokyo Institute of Technology B2-521 4259 Nagatsuta-cho Midori-ku Yokohama 226-8501 Japan; Department of Physics Faculty of Mathematics and Natural Sciences Bandung Institute of Technology Bandung 46132 Indonesia
Type
Article
Source Title
Nanomaterials
ISSN
20794991
Year
2023
Volume
13
Issue
21
Open Access
All Open Access Gold Green
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
DOI
10.3390/nano13212846
Abstract
Rapid and sensitive detection of Dengue virus remains a critical challenge in global public health. This study presents the development and evaluation of a Zinc Oxide nanorod (ZnO NR)-surface-integrated microfluidic platform for the early detection of Dengue virus. Utilizing a seed-assisted hydrothermal synthesis method high-purity ZnO NRs were synthesized characterized by their hexagonal wurtzite structure and a high surface-to-volume ratio offering abundant binding sites for bioconjugation. Further a comparative analysis demonstrated that the ZnO NR substrate outperformed traditional bare glass substrates in functionalization efficiency with 4G2 monoclonal antibody (mAb). Subsequent optimization of the functionalization process identified 4% (3-Glycidyloxypropyl)trimethoxysilane (GPTMS) as the most effective surface modifier. The integration of this substrate within a herringbone-structured microfluidic platform resulted in a robust device for immunofluorescence detection of DENV-3. The limit of detection (LOD) for DENV-3 was observed to be as low as 3.1 ? 10?4 ng/mL highlighting the remarkable sensitivity of the ZnO NR-integrated microfluidic device. This study emphasizes the potential of ZnO NRs and the developed microfluidic platform for the early detection of DENV-3 with possible expansion to other biological targets hence paving the way for enhanced public health responses and improved disease management strategies. ? 2023 by the authors.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
Scopus