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Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q Exactive Plus Orbitrap MS
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Metadata
Document Title
Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q Exactive Plus Orbitrap MS
Author
Ar-sanork K, Karuwan C, Surapanich N, Wilairat P, Nacapricha D, Chaisuwan P
Name from Authors Collection
Affiliations
Suranaree University of Technology; National Science & Technology Development Agency - Thailand; Rajabhat Rajanagarindra University; Mahidol University; Mahidol University; Mahidol University; Mahidol University
Type
Article
Source Title
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
Year
2021
Volume
12
Issue
1
Open Access
gold
Publisher
SPRINGER INTERNATIONAL PUBLISHING AG
DOI
10.1186/s40543-021-00275-5
Format
Abstract
This work developed a fast and simple method for the quantification of two important beta-agonists, ractopamine (RAC) and clenbuterol (CLEN) in animal urines. The method was based on micro-extraction by a mixed mode monolithic material and analysis by HPLC-UV and UHPLC-Q Exactive Plus Orbitrap mass spectrometer. A mixed functional monolith with nonpolar ester-based structure containing polar carboxylic groups was thermally synthesized in situ in the pipette tips. The material combines both hydrophobic and ionic interactions. Parameters including conditions and composition of reagents for the in-pipette tip monolith synthesis, as well as the extraction process, were investigated and optimized. The procedure for extraction is 800 mu L washing solvent of 10:90 (v/v) ACN:water and 150 mu L eluting solvent of 30:70 (v/v) ACN:200 mM acetate buffer pH 4.0. Extraction efficiencies of 92% and 100% for RAC and CLEN, respectively, were achieved within 5 min with total organic solvent consumption of 395 mu L. The extracts of spiked cattle and swine urines were analyzed by HPLC-UV and UHPLC-Q Exactive Plus Orbitrap mass spectrometer. Good recovery with acceptable precision was observed. The mass spectrometry data confirmed efficient matrix removal by the synthesized extraction sorbent allowing routine analysis by the HPLC-UV method.
Keyword
Clenbuterol | HPLC analysis | Micro-solid phase extraction | Monolith | Ractopamine
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Institute of Research and Development, Suranaree University of Technology, Thailand [IRD1-102-62-12-05]
Publication Source
WOS